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Loop nerve graft prefabrication for peripheral nerve defect reconstruction

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dc.contributor.authorŞİRVANCI, SERAP
dc.contributor.authorsOksuz S., Eren F., Cesur C., AÇIKEL ELMAS M., ŞİRVANCI S.
dc.date.accessioned2022-10-31T17:05:38Z
dc.date.available2022-10-31T17:05:38Z
dc.date.issued2022-08-01
dc.description.abstractBACKGROUND: Delayed autologous nerve graft reconstruction is inevitable in devastating injuries. Delayed or prolonged repair time has deleterious effects on nerve grafts. We aimed improving and accelerating nerve graft reconstruction process in a rat long nerve defect model with loop nerve graft prefabrication particularly to utilize for injuries with tissue loss. METHODS: Twenty-four Sprague-Dawley rats were allocated into three groups. 1.5 cm long peroneal nerve segment was excised, reversed in orientation, and used as autologous nerve graft. In conventional interpositional nerve graft group (Group 1), nerve defects were repaired in single-stage. In loop nerve graft prefabrication group (Group 2), grafts were sutured end-to-end (ETE) to the proximal peroneal nerve stumps. Distal ends of the grafts were sutured end-to-side to the peroneal nerve stumps 5 mm proximal to the ETE repair sites in first stage. In second stage, distal ends of the prefabricated grafts were transposed and sutured to distal nerve stumps. In staged conventional interpositional nerve graft group (Group 3), grafts were sutured ETE to proximal peroneal nerve stumps in first stage. Distal ends of the grafts and nerve stumps were tacked to the surrounding muscles until the final repair in second stage. Followup period was 4 weeks for each stage in Groups 2 and 3, and 8 weeks for Group 1. Peroneal function index (PFI), electrophysiology, and histological assessments were conducted after 8 weeks. P<0.05 was considered significant for statistical analysis. RESULTS: PFI results of Group 1 (−22.75±5.76) and 2 (−22.08±6) did not show statistical difference (p>0.05). Group 3 (−33.64±6.4) had a statistical difference compared to other groups (p<0.05). Electrophysiology results of Group 1 (16.19±2.15 mV/1.16±0.21 ms) and 2 (15.95±2.82 mV/1.17±0.16 ms) did not present statistical difference (p>0.05), whereas both groups had a statistical difference compared to Group 3 (10.44±1.96 mV/1.51±0.15 ms) (p<0.05). Axon counts of Group 1 (2227±260.4) and 3 (2194±201.1) did not have statistical difference (p>0.05), whereas both groups had significantly poor axon counts compared to Group 2 (2531±91.18) (p<0.05). CONCLUSION: Loop nerve graft prefabrication improved axonal regeneration without delay. Loop prefabrication can accelerate prolonged regeneration time for the injuries indicating a delayed nerve reconstruction. Higher axon counts derived with loop nerve prefabrication may even foster its investigation in immediate long nerve defect reconstructions in further studies.
dc.identifier.citationOksuz S., Eren F., Cesur C., AÇIKEL ELMAS M., ŞİRVANCI S., "Loop nerve graft prefabrication for peripheral nerve defect reconstruction", ULUSAL TRAVMA VE ACIL CERRAHI DERGISI-TURKISH JOURNAL OF TRAUMA & EMERGENCY SURGERY, cilt.28, sa.8, ss.1043-1051, 2022
dc.identifier.doi10.14744/tjtes.2022.68353
dc.identifier.endpage1051
dc.identifier.issn1306-696X
dc.identifier.issue8
dc.identifier.startpage1043
dc.identifier.urihttps://avesis.marmara.edu.tr/api/publication/9974fca3-b37f-4b2e-a371-e0a811245148/file
dc.identifier.urihttps://hdl.handle.net/11424/282703
dc.identifier.volume28
dc.language.isoeng
dc.relation.ispartofULUSAL TRAVMA VE ACIL CERRAHI DERGISI-TURKISH JOURNAL OF TRAUMA & EMERGENCY SURGERY
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectTıp
dc.subjectCerrahi Tıp Bilimleri
dc.subjectAcil Tıp
dc.subjectSağlık Bilimleri
dc.subjectMedicine
dc.subjectSurgery Medicine Sciences
dc.subjectEmergency Medicine
dc.subjectHealth Sciences
dc.subjectACİL TIP
dc.subjectKlinik Tıp
dc.subjectKlinik Tıp (MED)
dc.subjectEMERGENCY MEDICINE
dc.subjectCLINICAL MEDICINE
dc.subjectClinical Medicine (MED)
dc.subjectAcil Tıp Hizmetleri
dc.subjectEmergency Medical Services
dc.subjectLoop nerve prefabrication
dc.subjectnerve graft
dc.subjectnerve injury
dc.subjectnerve
dc.subjectTO-SIDE NEURORRHAPHY
dc.subjectSCHWANN-CELLS
dc.subjectREGENERATION
dc.subjectREPAIR
dc.subjectSTRATEGIES
dc.subjectLENGTH
dc.subjectLoop nerve prefabrication
dc.subjectnerve graft
dc.subjectnerve injury
dc.subjectnerve repair.
dc.titleLoop nerve graft prefabrication for peripheral nerve defect reconstruction
dc.typearticle
dspace.entity.typePublication
local.avesis.id9974fca3-b37f-4b2e-a371-e0a811245148
local.indexed.atWOS
local.indexed.atPUBMED
local.indexed.atSCOPUS
relation.isAuthorOfPublicationfcdb4c83-6090-4b1e-8758-448d3b32b73a
relation.isAuthorOfPublication.latestForDiscoveryfcdb4c83-6090-4b1e-8758-448d3b32b73a

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