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A genome-scale metabolic network of the aroma bacterium Leuconostoc mesenteroides subsp. cremoris

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dc.contributor.authorTOKSOY ÖNER, EBRU
dc.contributor.authorsOzcan, Emrah; Selvi, S. Selvin; Nikerel, Emrah; Teusink, Bas; Oner, Ebru Toksoy; Cakir, Tunahan
dc.date.accessioned2022-03-14T09:14:18Z
dc.date.available2022-03-14T09:14:18Z
dc.date.issued2019-04
dc.description.abstractLeuconostoc mesenteroides subsp. cremoris is an obligate heterolactic fermentative lactic acid bacterium that is mostly used in industrial dairy fermentations. The phosphoketolase pathway (PKP) is a unique feature of the obligate heterolactic fermentation, which leads to the production of lactate, ethanol, and/or acetate, and the final product profile of PKP highly depends on the energetics and redox state of the organism. Another characteristic of the L. mesenteroides subsp. cremoris is the production of aroma compounds in dairy fermentation, such as in cheese production, through the utilization of citrate. Considering its importance in dairy fermentation, a detailed metabolic characterization of the organism is necessary for its more efficient use in the industry. To this aim, a genome-scale metabolic model of dairy-origin L. mesenteroides subsp. cremoris ATCC 19254 (iLM.c559) was reconstructed to explain the energetics and redox state mechanisms of the organism in full detail. The model includes 559 genes governing 1088 reactions between 1129 metabolites, and the reactions cover citrate utilization and citrate-related flavor metabolism. The model was validated by simulating co-metabolism of glucose and citrate and comparing the in silico results to our experimental results. Model simulations further showed that, in co-metabolism of citrate and glucose, no flavor compounds were produced when citrate could stimulate the formation of biomass. Significant amounts of flavor metabolites (e.g., diacetyl and acetoin) were only produced when citrate could not enhance growth, which suggests that flavor formation only occurs under carbon and ATP excess. The effects of aerobic conditions and different carbon sources on product profiles and growth were also investigated using the reconstructed model. The analyses provided further insights for the growth stimulation and flavor formation mechanisms of the organism.
dc.identifier.doi10.1007/s00253-019-09630-4
dc.identifier.eissn1432-0614
dc.identifier.issn0175-7598
dc.identifier.pubmed30712128
dc.identifier.urihttps://hdl.handle.net/11424/242827
dc.identifier.wosWOS:000463607300023
dc.language.isoeng
dc.publisherSPRINGER
dc.relation.ispartofAPPLIED MICROBIOLOGY AND BIOTECHNOLOGY
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectLactic acid bacteria
dc.subjectLeuconostoc mesenteroides subsp
dc.subjectcremoris
dc.subjectHeterolactic fermentation
dc.subjectFlavor metabolism
dc.subjectGenome-scale metabolic model
dc.subjectFlux balance analysis
dc.subjectLACTIC-ACID BACTERIA
dc.subjectLACTOCOCCUS-LACTIS
dc.subjectLACTOBACILLUS-PLANTARUM
dc.subjectSTARTER CULTURES
dc.subjectGROWTH
dc.subjectGLUCOSE-6-PHOSPHATE-DEHYDROGENASE
dc.subjectCITRATE
dc.subjectFERMENTATION
dc.subjectPHYSIOLOGY
dc.subjectMECHANISM
dc.titleA genome-scale metabolic network of the aroma bacterium Leuconostoc mesenteroides subsp. cremoris
dc.typearticle
dspace.entity.typePublication
local.avesis.id017e46eb-e641-4f95-88a1-0485e6b1e11e
local.import.packageSS16
local.indexed.atWOS
local.indexed.atSCOPUS
local.indexed.atPUBMED
local.journal.numberofpages13
local.journal.quartileQ2
oaire.citation.endPage3165
oaire.citation.issue7
oaire.citation.startPage3153
oaire.citation.titleAPPLIED MICROBIOLOGY AND BIOTECHNOLOGY
oaire.citation.volume103
relation.isAuthorOfPublication6118e9e4-a58e-429b-bbec-4f73a3089a2b
relation.isAuthorOfPublication.latestForDiscovery6118e9e4-a58e-429b-bbec-4f73a3089a2b

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