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Effects of intra- and extracellular factors on anti-aging klotho gene expression

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dc.contributor.authorTURAN, KADİR
dc.contributor.authorsTuran, K.; Ata, P.
dc.date.accessioned2022-03-14T09:23:59Z
dc.date.available2022-03-14T09:23:59Z
dc.date.issued2011
dc.description.abstractInactivation of the klotho gene in mice causes serious systemic disorders, resembling human aging. However, at the molecular level, its action mechanisms are not well understood. The stimulatory or inhibitory effects of cis- and trans-regulatory factors on the klotho gene expression are also still unclear. We studied the effects of intra- and extracellular factors on human klotho gene expression. For this purpose, pHKP-Luc and pHKP-GFP reporter vectors were constructed with the 2.1-kbp upstream region of human klotho, covering its promoter region, using luciferase and GFP genes as the reporter. A series of vectors that have deletions in the upstream region of the klotho gene were constructed to assay cis-acting factors. Deletion of some parts of the klotho gene upstream region significantly affected reporter gene expression in HEK293 cells. p16 and p53 proteins inhibited reporter luciferase expression under the control of human klotho promoter in a dose-dependent manner. Calcium and phosphate ions stimulated klotho expression. p21, PTH, IGF-1, and angiotensin-II had no significant effect on klotho expression in HEK293 cells.
dc.identifier.doi10.4238/vol10-3gmr1261
dc.identifier.issn1676-5680
dc.identifier.pubmed21948763
dc.identifier.urihttps://hdl.handle.net/11424/243069
dc.identifier.wosWOS:000295823500071
dc.language.isoeng
dc.publisherFUNPEC-EDITORA
dc.relation.ispartofGENETICS AND MOLECULAR RESEARCH
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectklotho gene
dc.subjectAnti-aging
dc.subjectApoptosis
dc.subjectPTH
dc.subjectIGF-1
dc.subjectAngiotensin-II
dc.subjectTRANSCRIPTS ENCODING MEMBRANE
dc.subjectPHOSPHATE HOMEOSTASIS
dc.subjectOXIDATIVE STRESS
dc.subjectHORMONE KLOTHO
dc.subjectALPHA-KLOTHO
dc.subjectREGULATOR
dc.subjectSENESCENCE
dc.subjectMICE
dc.subjectCALCIUM
dc.subjectKIDNEY
dc.titleEffects of intra- and extracellular factors on anti-aging klotho gene expression
dc.typearticle
dspace.entity.typePublication
local.avesis.idc38a2f84-7a1c-4714-ba49-2dc622e16001
local.import.packageSS16
local.indexed.atWOS
local.indexed.atSCOPUS
local.indexed.atPUBMED
local.journal.numberofpages15
oaire.citation.endPage2023
oaire.citation.issue3
oaire.citation.startPage2009
oaire.citation.titleGENETICS AND MOLECULAR RESEARCH
oaire.citation.volume10
relation.isAuthorOfPublication9cc23fc2-5031-46cd-91a0-a2e13ab788d4
relation.isAuthorOfPublication.latestForDiscovery9cc23fc2-5031-46cd-91a0-a2e13ab788d4

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