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Il-6 and UGT1A1 variations may related to furosemide resistance in heart failure patients

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dc.contributor.authorGÜNEY, AHMET İLTER
dc.contributor.authorSÜNBÜL, MURAT
dc.contributor.authorsKoprululu Kucuk G., GÜNEY A. İ., SÜNBÜL M., Guctekin T., Koç G., Kirac D.
dc.date.accessioned2023-06-19T07:37:11Z
dc.date.available2023-06-19T07:37:11Z
dc.date.issued2023-01-01
dc.description.abstractFurosemide is a diuretic and is used for the treatment of patients with heart failure (HF). It has been found that in some HF patients, the drug does not treat patients efficiently. This condition is named as furosemide resistance. In this study, it is aimed to investigate the relationship between UDP-glucuronosyltransferase 1 (UGT1A1) and interleukine-6 (IL-6) variations with furosemide resistance in HF patients. Sixty HF patients using furosemide (patient group) and 30 healthy individuals (control group) were enrolled in this study. Patients were divided into two subgroups as non-responders (furosemide resistant) group (n = 30) and the responders (non-resistant) group (n = 30) according to the presence of furosemide resistance (n = 30). Variations in the first exon of UGT1A1 and rs1800795 and rs1800796 variations in IL-6 were analyzed by direct sequencing and real-time polymerase chain reaction (RT-PCR), respectively. The effects of newly detected mutations on 3-D protein structure were analyzed by in silico analysis. At the end of the study, 11 variations were detected in UGT1A1, of which nine of them are novel and eight of them cause amino acid change. Also, rs1800795 and rs1800796 variations were detected in all the groups. When patient and control groups were compared with each other, rs1800796 mutation in IL-6 was found statistically high in the patient group (p = 0.027). When the three groups were compared with each other, similarly, rs1800796 mutation in IL-6 was found statistically high in the non-responders group (p = 0.043). When allele distributions were compared between the patient and control groups, the C allele of rs1800795 mutation in IL-6 was found statistically high in the patient group (p = 0.032). When allele distributions were compared between the three groups, 55T-insertion in UGT1A1 was found statistically high in the non-responders group (p = 0.017). According to in silico analysis results, two variations were found deleterious and six variations were detected as probably damaging to protein functions. Our study may contribute to the elucidation of pharmacogenetic features (drug response–gene relationship) and the development of individual-specific treatment strategies in HF patients using furosemide.
dc.identifier.citationKoprululu Kucuk G., GÜNEY A. İ., SÜNBÜL M., Guctekin T., Koç G., Kirac D., "Il-6 and UGT1A1 variations may related to furosemide resistance in heart failure patients", IUBMB Life, 2023
dc.identifier.doi10.1002/iub.2732
dc.identifier.issn1521-6543
dc.identifier.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85161420327&origin=inward
dc.identifier.urihttps://hdl.handle.net/11424/290362
dc.relation.ispartofIUBMB Life
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectTıp
dc.subjectHistoloji-Embriyoloji
dc.subjectDahili Tıp Bilimleri
dc.subjectTıbbi Genetik
dc.subjectYaşam Bilimleri
dc.subjectMoleküler Biyoloji ve Genetik
dc.subjectSitogenetik
dc.subjectSağlık Bilimleri
dc.subjectTemel Tıp Bilimleri
dc.subjectTemel Bilimler
dc.subjectMedicine
dc.subjectHistology and Embryology
dc.subjectInternal Medicine Sciences
dc.subjectMedical Genetics
dc.subjectLife Sciences
dc.subjectMolecular Biology and Genetics
dc.subjectCytogenetic
dc.subjectHealth Sciences
dc.subjectFundamental Medical Sciences
dc.subjectNatural Sciences
dc.subjectYaşam Bilimleri (LIFE)
dc.subjectHÜCRE BİYOLOJİSİ
dc.subjectBİYOKİMYA VE MOLEKÜLER BİYOLOJİ
dc.subjectGENETİK VE KALITIM
dc.subjectLife Sciences (LIFE)
dc.subjectMOLECULAR BIOLOGY & GENETICS
dc.subjectCELL BIOLOGY
dc.subjectBIOCHEMISTRY & MOLECULAR BIOLOGY
dc.subjectGENETICS & HEREDITY
dc.subjectBiyokimya
dc.subjectMoleküler Biyoloji
dc.subjectGenetik
dc.subjectKlinik Biyokimya
dc.subjectHücre Biyolojisi
dc.subjectBiochemistry
dc.subjectMolecular Biology
dc.subjectGenetics
dc.subjectClinical Biochemistry
dc.subjectCell Biology
dc.subjectdiuretic resistance
dc.subjectfurosemide
dc.subjectheart failure
dc.subjectIL-6
dc.subjectUGT1A1
dc.subjectINTERLEUKIN-6 GENE POLYMORPHISMS
dc.subjectUGT1A1-ASTERISK-28 ALLELE
dc.subjectCARDIOVASCULAR-DISEASE
dc.subjectRISK
dc.subjectASSOCIATION
dc.subjectSUSCEPTIBILITY
dc.subjectPOTASSIUM
dc.subjectSMOKING
dc.subjectONSET
dc.titleIl-6 and UGT1A1 variations may related to furosemide resistance in heart failure patients
dc.typearticle
dspace.entity.typePublication
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local.indexed.atWOS
local.indexed.atPUBMED
local.indexed.atSCOPUS
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relation.isAuthorOfPublication.latestForDiscoveryd474fc8a-ae88-487e-b63b-7f506191fb94

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