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Preparation, characterization, and in vitro evaluation of isoniazid and rifampicin-loaded archaeosomes

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dc.contributor.authorOGAN, AYŞE
dc.contributor.authorsAttar, Azade; Bakir, Ceren; Yuce-Dursun, Basak; Demir, Serap; Cakmakci, Emrah; Danis, Ozkan; Birbir, Meral; Ogan, Ayse
dc.date.accessioned2022-03-12T22:25:33Z
dc.date.available2022-03-12T22:25:33Z
dc.date.issued2018
dc.description.abstractThe ability of Archaea to adapt their membrane lipid compositions to extreme environments has brought in archaeosomes into consideration for the development of drug delivery systems overcoming the physical, biological blockades that the body exhibits against drug therapies. In this study, we prepared unilamellar archaeosomes, from the polar lipid fraction extracted from Haloarcula 2TK2 strain, and explored its potential as a drug delivery vehicle. Rifampicin and isoniazid which are conventional drugs in tuberculosis medication were loaded separately and together in the same archaeosome formulation for the benefits of the combined therapy. Particle size and zeta potential of archaeosomes were measured by photon correlation spectroscopy, and the morphology was assessed by with an atomic force microscope. Encapsulation efficiency and loading capacities of the drugs were determined, and in vitro drug releases were monitored spectrophotometrically. Our study demonstrates that rifampicin and isoniazid could be successfully loaded separately and together in archaeosomes with reasonable drug-loading and desired vesicle-specific characters. Both of the drugs had greater affinity for archaeosomes than a conventional liposome formulation. The results imply that archaeosomes prepared from extremely halophilic archaeon were compatible with the liposomes for the development of stable and sustained release of antituberculosis drugs.
dc.identifier.doi10.1111/cbdd.13066
dc.identifier.eissn1747-0285
dc.identifier.issn1747-0277
dc.identifier.pubmed28667670
dc.identifier.urihttps://hdl.handle.net/11424/234939
dc.identifier.wosWOS:000418803800013
dc.language.isoeng
dc.publisherWILEY
dc.relation.ispartofCHEMICAL BIOLOGY & DRUG DESIGN
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectarchaeosome
dc.subjectdrug loading
dc.subjectisoniazid
dc.subjectliposome
dc.subjectrifampicin
dc.subjectDRUG-DELIVERY SYSTEMS
dc.subjectFORMULATION PARAMETERS
dc.subjectUNILAMELLAR LIPOSOMES
dc.subjectORAL DELIVERY
dc.subjectSTABILITY
dc.subjectRELEASE
dc.subjectLIPIDS
dc.subjectCARRIERS
dc.subjectMACROPHAGES
dc.subjectMECHANISMS
dc.titlePreparation, characterization, and in vitro evaluation of isoniazid and rifampicin-loaded archaeosomes
dc.typearticle
dspace.entity.typePublication
local.avesis.id561fe45c-19cb-4eb6-8ba1-36e648a5fcd7
local.import.packageSS17
local.indexed.atWOS
local.indexed.atSCOPUS
local.indexed.atPUBMED
local.journal.numberofpages9
local.journal.quartileQ3
oaire.citation.endPage161
oaire.citation.issue1
oaire.citation.startPage153
oaire.citation.titleCHEMICAL BIOLOGY & DRUG DESIGN
oaire.citation.volume91
relation.isAuthorOfPublication58db3332-a5f8-4224-a021-aeea795f52fa
relation.isAuthorOfPublication.latestForDiscovery58db3332-a5f8-4224-a021-aeea795f52fa

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