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Alpha-lipoic acid protects against hepatic ischemia-reperfusion injury in rats

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dc.contributor.authorDULUNDU, ENDER
dc.contributor.authorsDulundu, Ender; Ozel, Yahya; Topaloglu, Umit; Sehirli, Ozer; Ercan, Feriha; Gedik, Nursal; Sener, Goksel
dc.date.accessioned2022-03-12T17:33:07Z
dc.date.available2022-03-12T17:33:07Z
dc.date.issued2007
dc.description.abstractBackground and Aim: To evaluate the protective effect of alpha-lipoic acid in reducing oxidative damage after severe hepatic ischemia/reperfusion (IR) injury. Methods: Wistar albino rats were subjected to 45 min of hepatic ischemia, followed by 60 min reperfusion period. Lipoic acid (100 mg/kg i.p.) was administered 15 min prior to ischemia and immediately before reperfusion period. At the end of the reperfusion period aspartate aminotransferase (AST), alanine aminotransferase (ALT), lactate dehydrogenase (LDH) activity, and cytokine, TNF-alpha and IL-1 beta levels were determined in serum samples. Malondialdehyde (MDA), and glutathione (GSH) levels and myeloperoxidase (MPO) activity were determined in the liver tissue samples while formation of reactive oxygen species was monitored by using chemiluminescence ( CL) technique with luminol and lucigenin probes. Tissues were also analyzed histologically. Results: Serum ALT, AST, and LDH activities and TNF-alpha and IL-1 beta levels were elevated in the I/R group, while this increase was significantly lower in the group of animals treated concomitantly with lipoic acid. Hepatic GSH levels, significantly depressed by I/R, were elevated back to control levels in lipoic acid-treated I/R group. Furthermore, increases in tissue luminol and lucigen-in CL, MDA levels and MPO activity due to I/R injury were reduced back to control levels with lipoic acid treatment. Conclusion: Since lipoic acid administration alleviated the I/R-induced liver injury and improved the hepatic structure and function, it seems likely that lipoic acid with its antioxidant and oxidant-scavenging properties may be of potential therapeutic value in protecting the liver against oxidative injury due to ischemia-reperfusion. Copyright (c) 2007 S. Karger AG, Basel.
dc.identifier.doi10.1159/000098953
dc.identifier.eissn1423-0313
dc.identifier.issn0031-7012
dc.identifier.pubmed17259747
dc.identifier.urihttps://hdl.handle.net/11424/228776
dc.identifier.wosWOS:000245137500005
dc.language.isoeng
dc.publisherKARGER
dc.relation.ispartofPHARMACOLOGY
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectischemia/reperfusion
dc.subjectlipoic acid
dc.subjectcytokine
dc.subjectlipid peroxidation
dc.subjectmyeloperoxidase
dc.subjectREACTIVE OXYGEN
dc.subjectDIHYDROLIPOIC ACID
dc.subjectFREE-RADICALS
dc.subjectLIVER-INJURY
dc.subjectAGED RATS
dc.subjectANTIOXIDANT
dc.subjectMECHANISMS
dc.subjectMELATONIN
dc.subjectMYELOPEROXIDASE
dc.subjectINFLAMMATION
dc.titleAlpha-lipoic acid protects against hepatic ischemia-reperfusion injury in rats
dc.typearticle
dspace.entity.typePublication
local.avesis.idc15a5d0b-ba11-442c-b846-480a25d6a728
local.import.packageSS17
local.indexed.atWOS
local.indexed.atSCOPUS
local.journal.numberofpages8
oaire.citation.endPage170
oaire.citation.issue3
oaire.citation.startPage163
oaire.citation.titlePHARMACOLOGY
oaire.citation.volume79
relation.isAuthorOfPublicationedcd2255-94d5-4f56-b0c8-1385c55279b0
relation.isAuthorOfPublication.latestForDiscoveryedcd2255-94d5-4f56-b0c8-1385c55279b0

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