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Correlation between plasma ccfDNA, mtDNA changes, CTCs, and epithelial-mesenchymal transition in breast cancer patients undergoing NACT

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dc.contributor.authorPEKER EYÜBOĞLU, İREM
dc.contributor.authorGÜLLÜ AMURAN, GÖKÇE
dc.contributor.authorYUMUK, PERRAN FULDEN
dc.contributor.authorAKKİPRİK, MUSTAFA
dc.contributor.authorsÇelik B., PEKER EYÜBOĞLU İ., Koca S., Ümit Uğurlu M., Alan Ö., GÜLLÜ AMURAN G., AKIN TELLİ T., Yumuk F., AKKİPRİK M.
dc.date.accessioned2024-09-24T11:09:20Z
dc.date.available2024-09-24T11:09:20Z
dc.date.issued2024-01-01
dc.description.abstractBackground/aim: Breast cancer is the most prevalent cancer in women, emphasizing need for noninvasive blood biomarkers to aid in treatment selection. Previous studies have demonstrated elevated levels of plasma circulating cell-free DNA (ccfDNA) in breast cancer patients. Both ccfDNA and mitochondrial DNA (mtDNA) are fragments released into the bloodstream. In this study, we investigated effectiveness of ccfDNA and mtDNA as indicators of treatment response and explored their potential as monitoring biomarkers. Additionally, we compared these markers with circulating tumor cell (CTC) data and assessed their relationship with epithelialmesenchymal transition (EMT). Materials and methods: Thirty-six female breast cancer patients and 21 healthy females were included in the study. Quantitative polymerase chain reaction (qPCR) was performed on plasma samples to measure levels of ND1, ND4, ALU115, ALU247, and GAPDH, and DNA integrity was determined by calculating ratios of ALU247/ALU115 and ND4/ND1. Results: After treatment, patients had a significant decrease in ccfDNA levels and a significant increase in mtDNA copy number (mtDNAcn). However, there was no significant change in ccfDNA and mtDNA integrity. When comparing all groups, patients exhibited higher levels of ALU115 and ALU247 compared to controls. Moreover, patients demonstrated significantly lower ccfDNA integrity than controls. Conclusion: This study represents the first comprehensive investigation of plasma ccfDNA levels, mtDNAcn, and integrities collectively. Furthermore, it is the first study to explore the relationship between these markers and CTCs, cancer stem cell markers, treatment response, and metastatic status. Our findings suggest that plasma ccfDNA and mtDNA may serve as potential biomarkers for assessing chemotherapy response and can be employed alone or in combination with other biomarkers to monitor treatment efficacy in breast cancer patients. Key words: Breast cancer, ccfDNA, mtDNA, neoadjuvant therapy, EMT
dc.identifier.citationÇelik B., PEKER EYÜBOĞLU İ., Koca S., Ümit Uğurlu M., Alan Ö., GÜLLÜ AMURAN G., AKIN TELLİ T., Yumuk F., AKKİPRİK M., "Correlation between plasma ccfDNA, mtDNA changes, CTCs, and epithelial-mesenchymal transition in breast cancer patients undergoing NACT", Turkish Journal of Medical Sciences, cilt.54, sa.4, ss.652-665, 2024
dc.identifier.doi10.55730/1300-0144.5834
dc.identifier.endpage665
dc.identifier.issn1300-0144
dc.identifier.issue4
dc.identifier.startpage652
dc.identifier.urihttps://avesis.marmara.edu.tr/api/publication/ce412609-9c83-4bc5-949e-bcd5dfa5c04d/file
dc.identifier.urihttps://hdl.handle.net/11424/297836
dc.identifier.volume54
dc.language.isoeng
dc.relation.ispartofTurkish Journal of Medical Sciences
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectTıp
dc.subjectSağlık Bilimleri
dc.subjectTemel Tıp Bilimleri
dc.subjectMedicine
dc.subjectHealth Sciences
dc.subjectFundamental Medical Sciences
dc.subjectKlinik Tıp (Med)
dc.subjectKlinik Tıp
dc.subjectTıp Genel & Dahili
dc.subjectClinical Medicine (Med)
dc.subjectClinical Medicine
dc.subjectMedicine General & Internal
dc.subjectGenel Tıp
dc.subjectGeneral Medicine
dc.subjectBreast cancer
dc.subjectccfDNA
dc.subjectEMT
dc.subjectmtDNA
dc.subjectneoadjuvant therapy
dc.titleCorrelation between plasma ccfDNA, mtDNA changes, CTCs, and epithelial-mesenchymal transition in breast cancer patients undergoing NACT
dc.typearticle
dspace.entity.typePublication
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local.indexed.atPUBMED
local.indexed.atSCOPUS
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