Publication: Molecularly imprinted nanoparticles with recognition properties towards diphtheria toxin for ELISA applications
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Date
2022-11-01
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Abstract
Plastic antibodies can be used for in vitro neutralization of biomacromolecules with different fragments due to their potential in
separation, purification, chemical sensor, catalysis and drug production studies. These polymer nanoparticles with binding affinity
and selectivity comparable to natural antibodies were prepared
using functional monomer synthesis and copolymerization of
acrylic monomers via miniemulsion polymerization. As a result,
the in vitro cytotoxic effect from diphtheria toxin was reduced by
MIPs. In vitro imaging experiments of polymer nanoparticles (plastic antibodies) were performed to examine the interaction of
diphtheria toxin with actin filaments, and MIPs inhibited diphtheria toxin damage on actin filaments. The enzyme-linked
immunosorbent assay (ELISA) was performed with plastic antibodies labeled with biotin, and it was determined that plastic antibodies could also be used for diagnostic purposes. We report that
molecularly imprinted polymers (MIPs), which are biocompatible
polymer nanoparticles, can capture and reduce the effect of diphtheria toxic and its fragment A.
HIGHLIGHTS
Macromolecules can be imprinted by using their fragments as
template molecules.
MIPs gain an affinity for the template molecule by covalent
binding, non-covalent interactions or ligand interactions, as
well as the ability to bind, release and recognize the template molecule.
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Sağlık Bilimleri, Temel Bilimler, Health Sciences, Natural Sciences, Klinik Tıp (MED), Temel Bilimler (SCI), Doğa Bilimleri Genel, ÇOK DİSİPLİNLİ BİLİMLER, Clinical Medicine (MED), Natural Sciences (SCI), NATURAL SCIENCES, GENERAL, MULTIDISCIPLINARY SCIENCES, Multidisipliner, Multidisciplinary, Molecularly imprinted polymer, plastic antibody, diphtheria toxin, ELISA
Citation
Alkanlı S. S. , Dal Yöntem F., Yaşar M., Güven C., Kahraman M. V. , Kayaman Apohan N., Aktaş Z., Öncül M. O. , Ünlü A., Akçakaya H., "Molecularly imprinted nanoparticles with recognition properties towards diphtheria toxin for ELISA applications", JOURNAL OF BIOMATERIALS SCIENCE, POLYMER EDITION, cilt.1, ss.1-15, 2022