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Intravesical hyaluronic acid treatment improves bacterial cystitis and reduces cystitis-induced hypercontractility in rats

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dc.contributor.authorYEGEN, BERRAK
dc.contributor.authorsYildiz, Nurdan; Alpay, Harika; Tugtepe, Halil; Kumral, Zarife Nigar Ozdemir; Akakin, Dilek; Ilki, Arzu; Sener, Goksel; Yegen, Berrak C.
dc.date.accessioned2022-03-13T12:48:09Z
dc.date.available2022-03-13T12:48:09Z
dc.date.issued2015
dc.description.abstractObjectiveTo investigate the effect of intravesical hyaluronic acid on Escherichia coli-induced cystitis and cystitis-induced hypercontractility in rats. MethodsBacterial cystitis was induced in Wistar female rats by intravesical inoculation of E.coli. Isotonic saline was instilled in the control group (n=6). The rats were either non-treated, treated with gentamycin (4mg/kg, 5days) or treated intravesically with hyaluronic acid (0.5mL, 0.5%). On the eighth day, the bladder tissues were excised for histological examination, and the measurements of myeloperoxidase, superoxide dismutase and catalase activities. Contraction/relaxation responses to carbachol, isoprotrenol and papaverine were studied. ResultsTissue myeloperoxidase activity was increased, but superoxide dismutase and catalase activities were decreased in bacterial cystitis, while hyaluronic acid treatment reversed these changes. In the hyaluronic acid-treated group, healing of the uroepithelium was observed, while decreased inflammatory cell infiltration was obvious in gentamycin-treated group. E.coli-induced cystitis in all rats resulted in increased contraction responses to carbachol compared with controls (P<0.01). Treatment with hyaluronic acid, but not gentamycin, significantly (P<0.05) depressed hypercontractility at maximum carbachol concentrations. In all rats with cystitis, papaverine-induced relaxation was increased, whereas isoproterenol-induced relaxation curves were not different between the studied groups. ConclusionGentamycin treatment, despite its ameliorative effect on inflammation, had no impact on the contractile dysfunction of the injured bladder. Intravesical hyaluronic acid, in addition to its supportive role in the healing of the epithelium, seems to lower the increased threshold for contraction and to reduce oxidative stress. These findings support a potential role for hyaluronic acid in the treatment of bacterial cystitis.
dc.identifier.doi10.1111/iju.12752
dc.identifier.eissn1442-2042
dc.identifier.issn0919-8172
dc.identifier.pubmed25808602
dc.identifier.urihttps://hdl.handle.net/11424/238171
dc.identifier.wosWOS:000355835500020
dc.language.isoeng
dc.publisherWILEY
dc.relation.ispartofINTERNATIONAL JOURNAL OF UROLOGY
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectcystitis
dc.subjectdetrusor hypercontractility
dc.subjecthyaluronic acid
dc.subjectinflammation
dc.subjectrats
dc.subjectURINARY-TRACT-INFECTIONS
dc.subjectOXIDATIVE STRESS
dc.subjectINTERSTITIAL CYSTITIS
dc.subjectBLADDER
dc.subjectPREVENTION
dc.subjectINHIBITION
dc.subjectSULFATE
dc.subjectKIDNEY
dc.subjectINJURY
dc.titleIntravesical hyaluronic acid treatment improves bacterial cystitis and reduces cystitis-induced hypercontractility in rats
dc.typearticle
dspace.entity.typePublication
local.avesis.id19e1817a-24cc-4eac-b946-b82de5e8e332
local.import.packageSS17
local.indexed.atWOS
local.indexed.atSCOPUS
local.indexed.atPUBMED
local.journal.numberofpages6
oaire.citation.endPage603
oaire.citation.issue6
oaire.citation.startPage598
oaire.citation.titleINTERNATIONAL JOURNAL OF UROLOGY
oaire.citation.volume22
relation.isAuthorOfPublicatione4eaf9ac-f8dc-4e2b-b940-895cc906790d
relation.isAuthorOfPublication.latestForDiscoverye4eaf9ac-f8dc-4e2b-b940-895cc906790d

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