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Investigation of the efficacy of mesenchymal stem cell-derived conditioned medium on wound healing through scratch test and teer analysis

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dc.contributor.authorAKSU, MEHMET BURAK
dc.contributor.authorsAkkoç T., AKSU M. B.
dc.date.accessioned2023-10-09T12:21:27Z
dc.date.available2023-10-09T12:21:27Z
dc.date.issued2023-01-01
dc.description.abstractThe aim is to investigate how Umbilical Cord-Derived Mesenchymal Stem Cells conditioned medium (UC-MSC-CM) can promote wound healing and epithelial barrier function. UC-MSCs were differentiated into osteocytes, adipocytes, and chondrocytes to determine their multipotency. Flow cytometric analyses confirmed positive surface markers (CD90, CD73, CD105) and the absence of negative markers (CD34, CD11b, CD19, HLA_DR). We prepared conditioned medium (CM) from these cells. UC-MSC CM was tested in in vitro wound healing experiments using scratch assays and transepithelial electrical resistance measurements at 6th, 12th, and 24th hours to assess its regenerative effects. UC-MSCs were found to be multipotent in the characterization and differentiation analysis, demonstrating their potential for use in regenerative medicine. In our wound model design, UC-MSC-CM significantly accelerated the healing process 75% and 92.7% at 12 and 24 hours respectively compared to the control group. According to TEER analyzes epithelial barrier resistance showed 200 Ω.cm2 with UC-MSC-CM healing process at the 12th and 24th hours while the control group showed 145 Ω.cm2 and 185 Ω.cm2 at the same time points, respectively. The study demonstrates the potential of UC-MSCs in repairing epithelial barriers and wound injuries. The UC-MSC-CM accelerates wound closure as the healing process advances and enhances barrier integrity and permeability. These findings highlight UC-MSC-CM as a cell-free therapy for regenerative medicine and wound healing. Further research should focus on understanding the underlying mechanisms and optimizing its therapeutic applications to revolutionize wound management and treatment.
dc.identifier.citationAkkoç T., AKSU M. B., "Investigation of The Efficacy of Mesenchymal Stem Cell-Derived Conditioned Medium on Wound Healing Through Scratch Test and Teer Analysis", Journal of Research in Pharmacy, cilt.27, sa.5, ss.2135-2144, 2023
dc.identifier.doi10.29228/jrp.492
dc.identifier.endpage2144
dc.identifier.issn2630-6344
dc.identifier.issue5
dc.identifier.startpage2135
dc.identifier.urihttps://avesis.marmara.edu.tr/api/publication/3e3ee50e-5258-4cd9-a11a-d5624069ca7b/file
dc.identifier.urihttps://hdl.handle.net/11424/294312
dc.identifier.volume27
dc.language.isoeng
dc.relation.ispartofJournal of Research in Pharmacy
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectTemel Eczacılık Bilimleri
dc.subjectEczacılık
dc.subjectYaşam Bilimleri
dc.subjectSağlık Bilimleri
dc.subjectTemel Bilimler
dc.subjectBasic Pharmaceutics Sciences
dc.subjectPharmacology and Therapeutics
dc.subjectLife Sciences
dc.subjectHealth Sciences
dc.subjectNatural Sciences
dc.subjectYaşam Bilimleri (LIFE)
dc.subjectFarmakoloji ve Toksikoloji
dc.subjectFARMAKOLOJİ VE ECZACILIK
dc.subjectLife Sciences (LIFE)
dc.subjectPHARMACOLOGY & TOXICOLOGY
dc.subjectPHARMACOLOGY & PHARMACY
dc.subjectGenel Farmakoloji, Toksikoloji ve Eczacılık
dc.subjectFarmakoloji (tıbbi)
dc.subjectGeneral Pharmacology, Toxicology and Pharmaceutics
dc.subjectPharmacology (medical)
dc.subjectconditions medium
dc.subjectscratch assay
dc.subjectTEER analysis
dc.subjectUmbilical cord derived mesenchymal stem cell
dc.subjectwound healing
dc.titleInvestigation of the efficacy of mesenchymal stem cell-derived conditioned medium on wound healing through scratch test and teer analysis
dc.typearticle
dspace.entity.typePublication
local.avesis.id3e3ee50e-5258-4cd9-a11a-d5624069ca7b
local.indexed.atSCOPUS
relation.isAuthorOfPublicationf7de5699-7a5c-4496-93cf-d8daba79da4f
relation.isAuthorOfPublication.latestForDiscoveryf7de5699-7a5c-4496-93cf-d8daba79da4f

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