Publication: Curvularia lunata: A fungus for possible berberine transformation
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Date
2022-01-01
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Abstract
Abstract: The prevalence of multidrug-resistant microorganisms results in an
urgent need for the development of new antimicrobial agents or new treatment
strategies. In this sense, plants serve different alternatives. Berberine, a plantderived compound, is one of the alkaloids known to display antimicrobial
activity against several types of microorganisms, while its being a substrate of
various efflux pumps causes a decrease in its efficacy. Biotransformation
makes it possible to obtain novel or more effective compounds with only minor
structural modifications using enzyme systems. In this study,
biotransformation of berberine by Curvularia lunata was examined. The
working concentration of berberine was determined by observing the microbial
growth on agar plates. The concentration of residual berberine in the media was
analyzed by HPLC. In addition, laccase and beta-glucosidase enzyme activities
were followed for their possible roles during the biotransformation of
berberine. The results show that at the end of 14 days, C. lunata consumed 99%
and 87% of berberine with the initial concentrations of 0.35 mg/mL and 0.5
mg/mL, respectively. Enzyme activities were not affected significantly. Since
the concentration of berberine decreased, the biotransformation of berberine by
C. lunata could be mentioned. Monitoring of biotransformation products plays
a crucial role in discovering novel antimicrobial compounds and new valuable
molecules.
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Keywords
Biotransformation, Berberine, Curvularia lunata, Antimicrobial resistance
Citation
Yılmaz D., AVCI F. G., SARIYAR AKBULUT B., "Curvularia lunata: A fungus for possible berberine transformation", International Journal of Secondary Metabolite, cilt.9, sa.1, ss.66-73, 2022