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Effect of cDMEM media containing Ectoine on human periodontal ligament mesenchymal stem cell survival and differentiation

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dc.contributor.authorAKKOÇ, TUNÇ
dc.contributor.authorsTuncer Budanur D., Zibandeh N., Genc D., Gokalp M., KAŞALİ K., AKKOÇ T., Sepet E.
dc.date.accessioned2022-12-26T17:56:53Z
dc.date.available2022-12-26T17:56:53Z
dc.date.issued2018-06-01
dc.description.abstractBackground/Aim: Ectoine is an amino acid that can preserve osmotic balance and has more preservative activity than other osmoregulators. There are no published reports on the osmoregulators’ effects on viability or differentiation of dental stem cells. The aim of this study was to investigate the effect of Ectoine as a storage media on the viability and differentiation potential of human periodontal ligament mesenchymal stem cells (hPDLMSCs). Materials and Methods: hPDLMSCs were obtained from impacted third molar teeth. The cells were isolated, submitted to trilineage differentiation, and characterized by flow cytometer (FC). hPDLMSCs were incubated with different media containing Ectoine, complete DMEM (cDMEM), Ectoine+cDMEM, milk, and tap water for 2, 6, 12, and 24 h. The cells were analyzed by FC for viability, early-apoptosis, late apoptosis, and necrosis rates. Osteogenic and fibroblastic differentiation in hPDLMSCs were investigated by Alizarin red stain and vimentin expression. Results: All treated groups showed significant decreases in cell viability after 2 h. Significant increases were detected in the number of dead cells between 2 and 12 h in all groups except the Ectoine+cDMEM group. The deposition of mineral matrix nodules was significantly higher in cells cultured with Ectoine+cDMEM compared with the other media. Higher vimentin expressions were detected in cells cultured with cDMEM and Ectoine+cDMEM media, respectively. Conclusions: Ectoine added to cDMEM media, promoted cell survival plus osteogenic and fibroblastic differentiation of hPDLMSCs.
dc.identifier.citationTuncer Budanur D., Zibandeh N., Genc D., Gokalp M., KAŞALİ K., AKKOÇ T., Sepet E., "Effect of cDMEM media containing Ectoine on human periodontal ligament mesenchymal stem cell survival and differentiation", DENTAL TRAUMATOLOGY, cilt.34, sa.3, ss.188-200, 2018
dc.identifier.doi10.1111/edt.12396
dc.identifier.endpage200
dc.identifier.issn1600-4469
dc.identifier.issue3
dc.identifier.startpage188
dc.identifier.urihttps://hdl.handle.net/11424/284132
dc.identifier.volume34
dc.language.isoeng
dc.relation.ispartofDENTAL TRAUMATOLOGY
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectTıp
dc.subjectDiş Hekimliği
dc.subjectSağlık Bilimleri
dc.subjectMedicine
dc.subjectDentistry
dc.subjectHealth Sciences
dc.subjectDİŞ HEKİMLİĞİ, ORAL CERRAHİ VE TIP
dc.subjectKlinik Tıp
dc.subjectKlinik Tıp (MED)
dc.subjectDENTISTRY, ORAL SURGERY & MEDICINE
dc.subjectCLINICAL MEDICINE
dc.subjectClinical Medicine (MED)
dc.subjectPeriodontoloji
dc.subjectOrtodonti
dc.subjectAğız Cerrahisi
dc.subjectDiş Hijyeni
dc.subjectDişçilik Hizmetleri
dc.subjectDiş Hekimliği (çeşitli)
dc.subjectPeriodontics
dc.subjectOrthodontics
dc.subjectOral Surgery
dc.subjectDental Hygiene
dc.subjectDental Assisting
dc.subjectDentistry (miscellaneous)
dc.subjectGeneral Dentistry
dc.subjectcell viability
dc.subjectectoine
dc.subjectfibroblastic differentiation
dc.subjectosteogenic differentiation
dc.subjectperiodontal ligament cells
dc.subjectTRAUMATIC DENTAL INJURIES
dc.subjectSTORAGE MEDIA
dc.subjectIN-VITRO
dc.subjectAVULSED TEETH
dc.subjectGROWTH-FACTORS
dc.subjectSTROMAL CELLS
dc.subjectCOCONUT WATER
dc.subjectVIABILITY
dc.subjectMILK
dc.subjectPLASTICITY
dc.subjectcell viability
dc.subjectectoine
dc.subjectfibroblastic differentiation
dc.subjectosteogenic differentiation
dc.subjectperiodontal ligament cells
dc.titleEffect of cDMEM media containing Ectoine on human periodontal ligament mesenchymal stem cell survival and differentiation
dc.typearticle
dspace.entity.typePublication
local.avesis.id2afdf68c-9ae1-428c-b0fd-041829eb795c
local.indexed.atWOS
local.indexed.atPUBMED
local.indexed.atSCOPUS
relation.isAuthorOfPublicationdde3c531-d82d-492a-96eb-d6724483eaa1
relation.isAuthorOfPublication.latestForDiscoverydde3c531-d82d-492a-96eb-d6724483eaa1

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