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Lactose hydrolyzing activity of the lactase immobilized polycaprolactone and silk fibroin-based nanofiber and nitrocellulose membrane

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dc.contributor.authorAKBAY, TUĞBA
dc.contributor.authorsYılmaz-Karaoğlu S., Gürel-Gökmen B., AKBAY T.
dc.date.accessioned2023-07-27T08:39:55Z
dc.date.available2023-07-27T08:39:55Z
dc.date.issued2022-10-01
dc.description.abstract© 2022 Elsevier LtdThis study aimed to investigate the activity of lactase immobilized polycaprolactone and silk fibroin (PCL/SF)-based nanofiber and nitrocellulose membrane for the preparation of lactose-reduced milk. PCL/SF -based nanofiber was prepared by using the electrospinning method. The lactase enzyme was immobilized using the physical adsorption method on both surfaces, and then the optimum operating temperature and pH of the immobilized enzymes were determined. The efficiency of the immobilized lactase enzyme was determined in both goat and cow milk. The nutrient content of milk was also analyzed before and after the incubation of nanofiber and membrane with milk. The lactose hydrolysis efficiency of the lactase immobilized nitrocellulose membrane was found to be higher than the lactase immobilized PCL/SF -based nanofiber. Lactose was hydrolyzed 59% in cow milk and 87% in goat milk by using lactase immobilized nitrocellulose membrane. 42% of lactose was also hydrolyzed in cow milk and 21% was hydrolyzed in goat milk by using lactase immobilized PCL/SF-based nanofiber. However, the use of these two bioactive surfaces did not change the fat and protein composition of both cow and goat milk. In conclusion, lactase immobilized nitrocellulose membrane was found to be more advantageous in the production of lactose-reduced milk than the lactase immobilized polycaprolactone/silk fibroin nanofiber.
dc.identifier.citationYılmaz-Karaoğlu S., Gürel-Gökmen B., AKBAY T., "Lactose hydrolyzing activity of the lactase immobilized polycaprolactone and silk fibroin-based nanofiber and nitrocellulose membrane", Food Bioscience, cilt.49, 2022
dc.identifier.doi10.1016/j.fbio.2022.101828
dc.identifier.issn2212-4292
dc.identifier.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85134210768&origin=inward
dc.identifier.urihttps://hdl.handle.net/11424/291717
dc.identifier.volume49
dc.language.isoeng
dc.relation.ispartofFood Bioscience
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectTarımsal Bilimler
dc.subjectZiraat
dc.subjectGıda Mühendisliği
dc.subjectYaşam Bilimleri
dc.subjectMoleküler Biyoloji ve Genetik
dc.subjectSitogenetik
dc.subjectTemel Bilimler
dc.subjectMühendislik ve Teknoloji
dc.subjectAgricultural Sciences
dc.subjectAgriculture
dc.subjectFood Engineering
dc.subjectLife Sciences
dc.subjectMolecular Biology and Genetics
dc.subjectCytogenetic
dc.subjectNatural Sciences
dc.subjectEngineering and Technology
dc.subjectTarım ve Çevre Bilimleri (AGE)
dc.subjectYaşam Bilimleri (LIFE)
dc.subjectTarım Bilimleri
dc.subjectMoleküler Biyoloji ve Genetik
dc.subjectGIDA BİLİMİ VE TEKNOLOJİSİ
dc.subjectBİYOKİMYA VE MOLEKÜLER BİYOLOJİ
dc.subjectAgriculture & Environment Sciences (AGE)
dc.subjectLife Sciences (LIFE)
dc.subjectAGRICULTURAL SCIENCES
dc.subjectMOLECULAR BIOLOGY & GENETICS
dc.subjectFOOD SCIENCE & TECHNOLOGY
dc.subjectBIOCHEMISTRY & MOLECULAR BIOLOGY
dc.subjectYemek bilimi
dc.subjectBiyokimya
dc.subjectFood Science
dc.subjectBiochemistry
dc.subjectLactose reduced milk
dc.subjectImmobilization
dc.subjectBioactive surface
dc.subjectElectrospinning
dc.subjectLactose intolerance
dc.subjectBETA-GALACTOSIDASE
dc.subjectLIPASE
dc.subjectIMPACT
dc.titleLactose hydrolyzing activity of the lactase immobilized polycaprolactone and silk fibroin-based nanofiber and nitrocellulose membrane
dc.typearticle
dspace.entity.typePublication
local.avesis.id9fbb24e4-0fbf-4bbc-9c68-3762c3a93656
local.indexed.atWOS
local.indexed.atSCOPUS
relation.isAuthorOfPublication340aca72-e793-4abe-b0d6-2af5ca6013e3
relation.isAuthorOfPublication.latestForDiscovery340aca72-e793-4abe-b0d6-2af5ca6013e3

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