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Carvacrol enhances the antimicrobial potency of berberine in bacillus subtilis

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dc.contributor.authorSAYAR, NİHAT ALPAGU
dc.contributor.authorSARIYAR AKBULUT, BERNA
dc.contributor.authorsAtas B., Aksoy C. S., Avci F. G., SAYAR N. A., Ulgen K., ÖZKIRIMLI ÖLMEZ E., SARIYAR AKBULUT B.
dc.date.accessioned2023-04-03T10:26:17Z
dc.date.available2023-04-03T10:26:17Z
dc.date.issued2022-05-01
dc.description.abstractThe essential oil carvacrol from oregano displays a wide range of biological activities among which is found the inhibition of efflux pumps. Thus, using carvacrol, the current work undertook the effort to potentiate the antimicrobial activity of berberine, a natural product with limited antimicrobial efficacy due to its efflux. Following the selection of concentrations for the combinatorial treatments, guided by checkerboard microtiter plate assay and growth experiments, ethidium bromide accumulation assay was used to find that 25 mu g mL(-1) carvacrol displayed a weak efflux pump inhibitor character in Bacillus subtilis. Scanning electron microscopy images and cellular material leakage assays showed that carvacrol at this concentration neither altered the morphology nor the permeability of the membrane alone but when combined with 75 mu g mL(-1) berberine. Among the efflux pumps of different families found in B. subtilis, except for BmrA and Mdr, the increase in the expressional changes was striking, with Blt displaying similar to 4500-fold increase in expression under the combination treatment. Overall, the findings demonstrated that carvacrol potentiated the effect of berberine; however, not only multiple pumps but also different targets may be responsible for the observed activity.
dc.identifier.citationAtas B., Aksoy C. S., Avci F. G., SAYAR N. A., Ulgen K., ÖZKIRIMLI ÖLMEZ E., SARIYAR AKBULUT B., "Carvacrol Enhances the Antimicrobial Potency of Berberine in Bacillus subtilis", CURRENT MICROBIOLOGY, cilt.79, sa.5, 2022
dc.identifier.doi10.1007/s00284-022-02823-7
dc.identifier.issn0343-8651
dc.identifier.issue5
dc.identifier.urihttps://pubmed.ncbi.nlm.nih.gov/35303184/
dc.identifier.urihttps://hdl.handle.net/11424/288080
dc.identifier.volume79
dc.language.isoeng
dc.relation.ispartofCURRENT MICROBIOLOGY
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectYaşam Bilimleri
dc.subjectTemel Bilimler
dc.subjectLife Sciences
dc.subjectNatural Sciences
dc.subjectMikrobiyoloji
dc.subjectYaşam Bilimleri (LIFE)
dc.subjectMICROBIOLOGY
dc.subjectLife Sciences (LIFE)
dc.titleCarvacrol enhances the antimicrobial potency of berberine in bacillus subtilis
dc.typearticle
dspace.entity.typePublication
local.avesis.idb529d42a-e017-45c1-b7b1-d46049096417
local.indexed.atWOS
local.indexed.atPUBMED
local.indexed.atSCOPUS
relation.isAuthorOfPublication522d63b7-f599-4ef4-8936-16003fb70d7d
relation.isAuthorOfPublicationa9f127d3-8332-44dd-a532-34f3ef20bdb5
relation.isAuthorOfPublication.latestForDiscovery522d63b7-f599-4ef4-8936-16003fb70d7d

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