Publication:
Covalent Immobilization of alpha-Amylase onto UV-Curable Coating

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dc.contributor.authorKAHRAMAN, MEMET VEZİR
dc.contributor.authorsYarar, Uemran; Kahraman, Memet Vezir
dc.date.accessioned2022-03-12T17:35:56Z
dc.date.available2022-03-12T17:35:56Z
dc.date.issued2009
dc.description.abstractA UV-curable N-(4-sodiumsulfophenyl)maleimide monomer was synthesized, and its potential for enzyme binding was investigated. The bromine, which is used to activate the synthesized monomer for covalent attachments, has the advantage of giving reaction with the surface groups of enzyme under very mild conditions (0 degrees C, 30 min). In this procedure, sulfonyl bromide pendant monomer reacted with amino groups of the protein to form sulfonamide bonds. Polymeric support was prepaped by UV-curing technique. The water adsorption value was found to be less than 1%. The enzyme-bounding yield was found to be 68.18 +/- 4.20 mg/g monomer. The maximum activity was observed at pH 6.5. Immobilization did not change the pH-dependency of the enzyme activity. It was found that the optimum temperature for the free enzyme was similar to 30 degrees C, whereas it shifted to nearly 50 degrees C for the immobilized enzyme. Free enzyme lost its activity completely within 15 days. Immobilized enzyme lost only 30% of its activity in 30 days. (C) 2009 Wiley Periodicals, Inc. J Appl Polym Sci 114: 3716-3722, 2009
dc.identifier.doi10.1002/app.30442
dc.identifier.eissn1097-4628
dc.identifier.issn0021-8995
dc.identifier.urihttps://hdl.handle.net/11424/229221
dc.identifier.wosWOS:000270746000046
dc.language.isoeng
dc.publisherWILEY
dc.relation.ispartofJOURNAL OF APPLIED POLYMER SCIENCE
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectalpha-amylase
dc.subjectcovalent immobilization
dc.subjectUV curing coating
dc.subjectsulfon amide
dc.subjectCARBONATE
dc.subjectMEMBRANE
dc.subjectTOOL
dc.titleCovalent Immobilization of alpha-Amylase onto UV-Curable Coating
dc.typearticle
dspace.entity.typePublication
local.avesis.idae2728a6-7875-4397-9096-3f3fac7618fd
local.import.packageSS17
local.indexed.atWOS
local.indexed.atSCOPUS
local.journal.numberofpages7
oaire.citation.endPage3722
oaire.citation.issue6
oaire.citation.startPage3716
oaire.citation.titleJOURNAL OF APPLIED POLYMER SCIENCE
oaire.citation.volume114
relation.isAuthorOfPublication7676f3ad-0384-4001-b17d-b928ae2de7e6
relation.isAuthorOfPublication.latestForDiscovery7676f3ad-0384-4001-b17d-b928ae2de7e6

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