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Ultrastructural GABA immunogold labeling in the substantia nigra pars reticulata of kindled genetic absence epilepsy rats

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dc.contributor.authorAKAKIN, DİLEK
dc.contributor.authorsSirvanci, Serap; Akakin, Dilek; Idrizoglu, Medine Gulcebi; Kaya, Ozlem Tugce; Karamahmutoglu, Tugba; Asik, Zehra Nur Turgan; Onat, Filiz
dc.date.accessioned2022-03-12T22:40:09Z
dc.date.available2022-03-12T22:40:09Z
dc.date.issued2020
dc.description.abstractGenetic Absence Epilepsy Rats from Strasbourg (GAERS) is a well-known animal model of absence epilepsy and they are resistant to electrical kindling stimulations. The present study aimed to examine possible differences in gamma-aminobutyric acid (GABA) levels and synapse counts in the substantia nigra pars reticulata anterior (SNRa) and posterior (SNRp) regions between GAERS and Wistar rats receiving kindling stimulations. Animals in the kindling group either received six stimulations in the amygdala and had grade 2 seizures or they were kindled, having grade five seizures. Rats were decapitated one hour after the last stimulation. SNR regions were obtained after vibratome sectioning of the brain tissue. GABA immunoreactivity was detected by immunogold method and synapses were counted. Sections were observed by transmission electron microscope and analyzed by Image J program. GABA density in the SNRa region of fully kindled GAERS and Wistar groups increased significantly compared to that of their corresponding grade 2 groups. The number of synapses increased significantly in kindled and grade 2 GAERS groups, compared to kindled and grade 2 Wistar groups, respectively, in the SNRa region. GABA density in the SNRp region of kindled GAERS group increased significantly compared to that of GAERS grade 2 group. In the SNRp region, both kindled and grade 2 GAERS groups were found to have increased number of synapses compared to that of GAERS control group. We concluded that both SNRa and SNRp regions may be important in modulating resistance of GAERS to kindling stimulations.
dc.identifier.doi10.1080/01913123.2020.1839153
dc.identifier.eissn1521-0758
dc.identifier.issn0191-3123
dc.identifier.pubmed33118420
dc.identifier.urihttps://hdl.handle.net/11424/235908
dc.identifier.wosWOS:000584805700001
dc.language.isoeng
dc.publisherTAYLOR & FRANCIS INC
dc.relation.ispartofULTRASTRUCTURAL PATHOLOGY
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectGAERS
dc.subjectkindling
dc.subjectelectron microscopy
dc.subjectimmunocytochemistry
dc.subjectGABAERGIC CELL-LINE
dc.subjectINTRANIGRAL TRANSPLANTS
dc.subjectELECTRICAL-STIMULATION
dc.subjectSEIZURE SUPPRESSION
dc.subjectRESISTANCE
dc.subjectMODEL
dc.subjectINACTIVATION
dc.subjectMECHANISMS
dc.subjectNEURONS
dc.titleUltrastructural GABA immunogold labeling in the substantia nigra pars reticulata of kindled genetic absence epilepsy rats
dc.typearticle
dspace.entity.typePublication
local.avesis.id5314a921-4f63-48cf-b892-9adca5f896c6
local.import.packageSS17
local.indexed.atWOS
local.indexed.atSCOPUS
local.indexed.atPUBMED
local.journal.numberofpages8
local.journal.quartileQ4
oaire.citation.endPage386
oaire.citation.issue4-6
oaire.citation.startPage379
oaire.citation.titleULTRASTRUCTURAL PATHOLOGY
oaire.citation.volume44
relation.isAuthorOfPublicationc97c0ebf-ffce-4b27-a933-941a53767b0d
relation.isAuthorOfPublication.latestForDiscoveryc97c0ebf-ffce-4b27-a933-941a53767b0d

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