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Interplay of adaptive capabilities of Halomonas sp AAD12 under salt stress

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dc.contributor.authorSARIYAR AKBULUT, BERNA
dc.contributor.authorsCeylan, Selim; Yilan, Gulsah; Akbulut, Berna Sariyar; Poli, Annarita; Kazan, Dilek
dc.date.accessioned2022-03-12T18:06:21Z
dc.date.available2022-03-12T18:06:21Z
dc.date.issued2012
dc.description.abstractIn the present study, osmoadaptive mechanism of Halomonas sp. AAD12 was studied through analysis of changes in its proteome maps and osmolyte accumulation strategy to understand how this euryhaline microorganism masters osmotic stress of saline environments. Under salt stress, there were significant variations in the expression of proteins involved in osmoregulation, stress response, energy generation and transport. This was accompanied by an increase in proline and hydroxyectoine but a decrease in ectoine accumulation. The major osmolyte at high salinity was proline. Unexpectedly the size of the total ectoines' pool was smaller at elevated salinity. Experimental findings were then integrated with a metabolic model to get insight into carbon trafficking during osmoadaptation. Simulations predicted that the total flux through energy generating pathways, namely gluconeogenesis and the pentose phosphate pathway, was significantly lower and carbon source that entered the system as citrate was mainly diverted to osmolyte synthesis at high salinity. Overall these results suggested that the moderately halophilic Halomonas sp. AAD12 pursued a different osmoregulatory strategy than the two well known moderate halophiles, Chromohalobacter salexigens and Halobacillus halophilus. The climbing value of osmolytes such as ectoine in health care and skin care products places significant attention to halophilic microorganisms hence an understanding of the osmoadaptive mechanism and osmolyte accumulation strategy of this isolate is very valuable to be able to manipulate its metabolism towards desired goals. (C) 2012, The Society for Biotechnology, Japan. All rights reserved.
dc.identifier.doi10.1016/j.jbiosc.2012.02.030
dc.identifier.eissn1347-4421
dc.identifier.issn1389-1723
dc.identifier.pubmed22575437
dc.identifier.urihttps://hdl.handle.net/11424/230887
dc.identifier.wosWOS:000307154300008
dc.language.isoeng
dc.publisherSOC BIOSCIENCE BIOENGINEERING JAPAN
dc.relation.ispartofJOURNAL OF BIOSCIENCE AND BIOENGINEERING
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectModerate halophile
dc.subjectProteome
dc.subjectOsmolyte
dc.subjectCarbon trafficking
dc.subjectOsmoadaptation
dc.subjectESCHERICHIA-COLI
dc.subjectMETABOLIC CAPABILITIES
dc.subjectPROTEOMIC ANALYSIS
dc.subjectBULK PROTEIN
dc.subjectHALOTOLERANT
dc.subjectADAPTATION
dc.subjectCHAPERONE
dc.subjectCLEAVAGE
dc.subjectGROWTH
dc.subjectOSMOADAPTATION
dc.titleInterplay of adaptive capabilities of Halomonas sp AAD12 under salt stress
dc.typearticle
dspace.entity.typePublication
local.avesis.ide21c041b-7c11-4fe5-b877-6d2efa027064
local.import.packageSS17
local.indexed.atWOS
local.indexed.atSCOPUS
local.indexed.atPUBMED
local.journal.numberofpages8
oaire.citation.endPage52
oaire.citation.issue1
oaire.citation.startPage45
oaire.citation.titleJOURNAL OF BIOSCIENCE AND BIOENGINEERING
oaire.citation.volume114
relation.isAuthorOfPublicationa9f127d3-8332-44dd-a532-34f3ef20bdb5
relation.isAuthorOfPublication.latestForDiscoverya9f127d3-8332-44dd-a532-34f3ef20bdb5

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