Publication:
Effect of Bumetanide on Facial Nerve Regeneration in Rat Model

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dc.contributor.authorAKAKIN, DİLEK
dc.contributor.authorsLongur, Ecem Sevim; Yigit, Ozgur; Ertugay, Cigdem Kalaycik; Server, Ela Araz; Adatepe, Turgut; Akakin, Dilek; Orun, Oya; Koroglu, Ayca Karagoz
dc.date.accessioned2022-03-12T22:44:32Z
dc.date.available2022-03-12T22:44:32Z
dc.date.issued2021
dc.description.abstractObjective We investigated the effects of bumetanide alone and in combination with dexamethasone on facial nerve regeneration in rats with facial paralysis. Study Design A prospective controlled animal study. Setting An animal laboratory. Subjects and Methods Facial paralysis was induced in 32 Wistar rats that we then divided into 4 groups: group 1, control; group 2, bumetanide; group 3, dexamethasone; group 4, bumetanide and dexamethasone. Electroneurography was performed 1, 2, and 4 weeks later, and nerve regeneration was evaluated by electron and light microscopy and Western blotting in week 4. Results Regarding the comparison between preoperative values and week 4, the latency difference in group 1 (1.25 milliseconds) was significantly higher than those of groups 2 to 4 (0.56, 0.34, and 0.10 milliseconds, respectively;P= .001). The latency increment in groups 2 and 3 was higher than that of group 4 (P= .002 andP= .046) in week 4, whereas groups 2 and 3 did not differ significantly (P= .291). Amplitude difference was not statistically significant from week 4 among all groups (allP >.05). The number of myelinated axons was significantly higher in all treatment groups than in the control group (P= .001). Axon number and intensity were significantly higher in group 4 as compared with groups 2 and 3 (P= .009,P= .005). Conclusion After primary neurorrhaphy, dexamethasone and bumetanide alone promoted nerve recovery based on electrophysiologic and histologic measures. Combination therapy was, however, superior.
dc.identifier.doi10.1177/0194599820937670
dc.identifier.eissn1097-6817
dc.identifier.issn0194-5998
dc.identifier.pubmed32600218
dc.identifier.urihttps://hdl.handle.net/11424/236441
dc.identifier.wosWOS:000544685400001
dc.language.isoeng
dc.publisherSAGE PUBLICATIONS LTD
dc.relation.ispartofOTOLARYNGOLOGY-HEAD AND NECK SURGERY
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectaquaporin
dc.subjectbumetanide
dc.subjectfacial paralysis
dc.subjectregeneration
dc.subjectSPINAL-CORD-INJURY
dc.subjectUP-REGULATION
dc.subjectMOUSE MODEL
dc.subjectRECOVERY
dc.subjectGROWTH
dc.subjectCORTICOSTEROIDS
dc.subjectINHIBITION
dc.subjectEXPRESSION
dc.subjectMETHYLPREDNISOLONE
dc.subjectAGENTS
dc.titleEffect of Bumetanide on Facial Nerve Regeneration in Rat Model
dc.typearticle
dspace.entity.typePublication
local.avesis.id025a29a7-5ee8-46af-81b2-d10413bfd3a8
local.import.packageSS17
local.indexed.atWOS
local.indexed.atSCOPUS
local.indexed.atPUBMED
local.journal.articlenumber194599820937670
local.journal.numberofpages7
local.journal.quartileQ1
oaire.citation.endPage123
oaire.citation.issue1
oaire.citation.startPage117
oaire.citation.titleOTOLARYNGOLOGY-HEAD AND NECK SURGERY
oaire.citation.volume164
relation.isAuthorOfPublicationc97c0ebf-ffce-4b27-a933-941a53767b0d
relation.isAuthorOfPublication.latestForDiscoveryc97c0ebf-ffce-4b27-a933-941a53767b0d

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