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The effects of riboflavin on ischemia/reperfusion induced renal injury: Role on caspase-3 expression

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dc.contributor.authorERTAŞ, BÜŞRA
dc.contributor.authorsAyaz Adakul, Betul; Ertas, Busra; Cevikelli, Zatiye Ayca; Ozbeyli, Dilek; Ercan, Feriha; Kandemir, Cansu; Cevik, Ozge; Sener, Tarik Emre; Sener, Goksel
dc.date.accessioned2022-03-14T09:17:09Z
dc.date.available2022-03-14T09:17:09Z
dc.date.issued2019-05-15
dc.description.abstractReactive oxygen metabolites play important roles in ischemia/reperfusion (I/R) injury in several organ systems. Riboflavin has been shown to exert antioxidant and/or anti-inflammatory activities in several experimental models. The aim of this study was to investigate the role of riboflavin against I/R injury in the rat kidney. Wistar albino rats 200-300 g weighing were divided into 3 groups. One week after unilateral nephrectomy, the IR procedure was applied to the rats. To induce I/R injury renal pedicle was clamped for 45 minutes and then rats were allowed reperfusion for 6 hours. Riboflavin (25 mg/ kg, orally) or vehicle was administered for one week as pretreatment. After decapitation, kidney tissue samples were taken for the evaluation of malondialdehyde (MDA), an end product of lipid peroxidation; glutathione (GSH), a key antioxidant; and 8-hydroxydeoxyguanosine (8-OHdG), a specific marker of oxidative DNA damage. Furthermore, myeloperoxidase (MPO) and caspase-3 activities were also examined together with histological analysis. Ischemia/reperfusion induced significant increases in MDA and 8-OHdG levels and MPO and caspase- 3 activities, and decrese in GSH levels. In the riboflavin treatment these indices were found to be reversed back to control levels. The present data demonstrated that riboflavin, through its antioxidant effect, attenuates I/R induced acute renal injury in rats.
dc.identifier.doi10.12991/jrp.2019.145
dc.identifier.issn2630-6344
dc.identifier.urihttps://hdl.handle.net/11424/242908
dc.identifier.wosWOS:000468157000004
dc.language.isoeng
dc.publisherMARMARA UNIV
dc.relation.ispartofJOURNAL OF RESEARCH IN PHARMACY
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectAntioxidants
dc.subjectischemia reperfusion
dc.subjectriboflavin
dc.subjectcaspase
dc.subjectABDOMINAL AORTIC-ANEURYSM
dc.subjectVITAMIN B-2
dc.subjectOXIDATIVE STRESS
dc.subjectRAT
dc.subjectREPERFUSION
dc.subjectISCHEMIA
dc.subjectMELATONIN
dc.subjectMYELOPEROXIDASE
dc.titleThe effects of riboflavin on ischemia/reperfusion induced renal injury: Role on caspase-3 expression
dc.typearticle
dspace.entity.typePublication
local.avesis.id63bbb173-1f96-4545-8ba3-9c09659dad11
local.import.packageSS16
local.indexed.atWOS
local.indexed.atSCOPUS
local.indexed.atTRDIZIN
local.journal.numberofpages8
oaire.citation.endPage386
oaire.citation.issue3
oaire.citation.startPage379
oaire.citation.titleJOURNAL OF RESEARCH IN PHARMACY
oaire.citation.volume23
relation.isAuthorOfPublication864e7cb0-b39f-48bb-a546-ee65ffc807b2
relation.isAuthorOfPublication.latestForDiscovery864e7cb0-b39f-48bb-a546-ee65ffc807b2

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