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Development and Validation of Liquid Chromatographic Method with DAD for the Quantification of Natamycin In Dairy Products

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dc.contributor.authorBİLĞİÇ ALKAYA, DİLEK
dc.contributor.authorsKaralomlu, Ozlem; Alkaya, Dilek Bilgic
dc.date.accessioned2022-03-14T08:23:53Z
dc.date.available2022-03-14T08:23:53Z
dc.date.issued2017-04-01
dc.description.abstractIn this paper high performance liquid chromatography method was developed for the determination of natamycin in dairy product in Turkish market. Chromatographic separation was achieved on a C8 column (150 mm x 4.6 mm x 5 mu m 100 mm) with a mobile phase of methanol : water : acetic acid (12:8:1 v/v/v), at 1ml/min flow rate with DAD detection at 303 nm. Natamycin was analysed simultaneously using HPLC-UV method of ISO 9233+2, 2007(IDF 140-2, 2007). The method was found linear over the concentration ranges of 0.01-0.8 mg/L. Correlation coefficients (r) of the regression equations were greater than 0.999. The LOD and LOQ values for UV and DAD were found 0.321 mg/kg and 0.355 mg/kg; 0.321 mg/kg and 0.388 mg/kg for cheese samples respectively. The relative recoveries obtained for the spiked cheese samples were between 90 and 110 %. Also the interday precision of the method was found as 1.874% and 3.442 % RSD values for UV and DAD dedections. From the results of the present study using HPLC-DAD indicated that it is not reasonable to attribute the determination of natamycin in cheese. Therefore, the difference of the validation parameters were found insignificant for both detections. However, It was succesfully applied to determination of 46 different cheese products and finding dealing with the presence of natamycin in cheese samples were presented.
dc.identifier.doi10.12991/marupj.300891
dc.identifier.issn1309-0801
dc.identifier.urihttps://hdl.handle.net/11424/241702
dc.identifier.wosWOS:000411289700020
dc.language.isoeng
dc.publisherMARMARA UNIV, FAC PHARMACY
dc.relation.ispartofMARMARA PHARMACEUTICAL JOURNAL
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectHPLC-DAD
dc.subjectvalidation
dc.subjectnatamycin
dc.subjectantifungal agent
dc.subjectCHEESE
dc.titleDevelopment and Validation of Liquid Chromatographic Method with DAD for the Quantification of Natamycin In Dairy Products
dc.typearticle
dspace.entity.typePublication
local.avesis.id1ff6fdc5-917a-4c73-8217-b4c53e83ba56
local.import.packageSS16
local.indexed.atWOS
local.indexed.atTRDIZIN
local.journal.numberofpages6
oaire.citation.endPage360
oaire.citation.issue2
oaire.citation.startPage355
oaire.citation.titleMARMARA PHARMACEUTICAL JOURNAL
oaire.citation.volume21
relation.isAuthorOfPublicationb3f0ae46-715d-4b45-bf08-49abdc4adbf4
relation.isAuthorOfPublication.latestForDiscoveryb3f0ae46-715d-4b45-bf08-49abdc4adbf4

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