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Fabrication of tissue-engineered tympanic membrane patches using 3D-Printing technology

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dc.contributor.authorŞAHİN, ALİ
dc.contributor.authorsIlhan, Elif; Ulag, Songul; Sahin, Ali; Yilmaz, Betul Karademir; Ekren, Nazmi; Kilic, Osman; Sengor, Mustafa; Kalaskar, Deepak M.; Oktar, Faik Nuzhet; Gunduz, Oguzhan
dc.date.accessioned2022-03-12T22:58:04Z
dc.date.available2022-03-12T22:58:04Z
dc.date.issued2021
dc.description.abstractIn recent years, scaffolds produced in 3D printing technology have become more widespread tool due to providing more advantages than traditional methods in tissue engineering applications. In this research, it was aimed to produce patches for the treatment of tympanic membrane perforations which caused significant hearing loss by using 3D printing method. Polylactic acid(PLA) scaffolds with Chitosan(CS) and Sodium Alginate(SA) added in various ratios were prepared for artificial eardrum patches. Different amounts of chitosan and sodium alginate added to PLA increased the biocompatibility of the produced scaffolds. The created patches were designed by mimicking the thickness of the natural tympanic membrane thanks to the precision provided by the 3D printed method. The produced scaffolds were analyzed separately for chemical, morphological, mechanical and biocompatibility properties. Scanning electron microscope (SEM), Fourier-transform infrared (FT-IR) spectroscopy was performed to observe the surface morphology and chemical structure of the scaffolds. Mechanical, thermal and physical properties, swelling and degradation behaviors were examined to fully analyze whole characteristic features of the samples. Cell culture study was also performed to demonstrate the biocompatibility properties of the fabricated scaffolds with human adipose tissue-derived mesenchymal stem cells (hAD-MSCs). 15 wt % PLA was selected as the control group and among all concentrations of CS and SA, groups containing 3 wt% CS and 3 wt% SA showed significantly superior and favorable features in printing quality. The research continued with these two scaffolds (3 wt% CS, and 3 wt% SA), which showed improved print quality when added to PLA. Overall, these results show that PLA/CS and PLA/SA 3D printed artificial patches have the potential to tissue engineering solutions to repair tympanic membrane perforation for people with hearing loss.
dc.identifier.doi10.1016/j.jmbbm.2020.104219
dc.identifier.eissn1878-0180
dc.identifier.issn1751-6161
dc.identifier.pubmed33302170
dc.identifier.urihttps://hdl.handle.net/11424/237138
dc.identifier.wosWOS:000608178400010
dc.language.isoeng
dc.publisherELSEVIER
dc.relation.ispartofJOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subject3D printing
dc.subjectBiomaterials
dc.subjectTissue engineering
dc.subjectTympanic membrane
dc.subjectArtificial eardrum patch
dc.subjectPOLYLACTIC ACID PLA
dc.subjectSCAFFOLDS
dc.subjectREPAIR
dc.subjectPERFORATIONS
dc.subjectDEGRADATION
dc.subjectCHITOSAN
dc.subjectPOROSITY
dc.subjectDESIGN
dc.titleFabrication of tissue-engineered tympanic membrane patches using 3D-Printing technology
dc.typearticle
dspace.entity.typePublication
local.avesis.idee72ad33-c655-45bf-a571-205530b93d7e
local.import.packageSS17
local.indexed.atWOS
local.indexed.atSCOPUS
local.indexed.atPUBMED
local.journal.articlenumber104219
local.journal.numberofpages12
oaire.citation.titleJOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS
oaire.citation.volume114
relation.isAuthorOfPublicationdda71138-8ce4-4265-89b2-73bc94786a4f
relation.isAuthorOfPublication.latestForDiscoverydda71138-8ce4-4265-89b2-73bc94786a4f

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