Publication: Evaluation of the effects of donepezil, memantine and α-lipoic acid combined administration in amnesia rats on impaired cognitive functions in terms of behavioural, apoptotic, cholinergic and glutamatergic systems
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Date
2021-12-01
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Abstract
Objective: The aim of the study was to evaluate the possible protective effect of donepezil, memantine and alpha-lipoic acid (α-LA) combined
therapy in the scopolamine-induced amnesia rat model.
Methods: In this study, the effect of combined therapy used in the treatment of scopolamine-induced amnesia on behavioural parameters
was evaluated using Y-maze and new object recognition (NOR) test. In addition, muscarinic acetylcholine receptor subtype M1, N-methyl-Daspartate receptor NR2B subunit, brain-derived neurotrophic factor (BDNF) and mitochondrial apoptosis-related proteins [B-cell lymphoma-2
(Bcl-2) / Bcl-2 associated X (Bax) ratio, caspase (casp) – 3, and – 9] expression levels were evaluated using the western blot method in the frontal
cortex and hippocampus regions.
Results: The main findings of this study demonstrated that in scopolamine-induced amnesia rats, cognitive dysfunction determined by both the
Y-maze and the NOR test were reversed with the combined treatment of memantine, donepezil and α-LA. According to immunoblotting results
in both brain regions, scopolamine-induced decreased M1, BDNF, Bcl-2 / Bax ratio and increased NR2B, casp-3 and – 9 expression levels were
found to be reversed to almost control values with combined treatment.
Conclusion: Consistent with the literature, our study results suggest that the positive contribution of α-LA to the combined treatment of
donepezil and memantine, which is used in the routine treatment of neurodegenerative diseases, may be a treatment option in the future.
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Tıp, Sağlık Bilimleri, Dahili Tıp Bilimleri, Tıbbi Ekoloji ve Hidroklimatoloji, Medicine, Health Sciences, Internal Medicine Sciences, Medical Ecology and Hydroclimatology, TIP, ARAŞTIRMA VE DENEYSEL, Klinik Tıp, Klinik Tıp (MED), MEDICINE, RESEARCH & EXPERIMENTAL, CLINICAL MEDICINE, Clinical Medicine (MED), İncelemeler ve Referanslar (tıbbi), Araştırma ve Teori, Reviews and References (medical), Research and Theory, Alpha-lipoic acid, amnesia, apoptosis, scopolamine, Y-maze, INDUCED MEMORY IMPAIRMENT, NEUROTROPHIC FACTOR, MUSCARINIC RECEPTORS, OXIDATIVE STRESS, SUBTYPES, MODEL, INFLAMMATION, ANTIOXIDANT, HIPPOCAMPUS, MECHANISMS, Alpha-lipoic acid, amnesia, apoptosis, scopolamine, Y-maze
Citation
Aykac A., ÖZBEYLİ D., Pekol G., Sehirli A. O., "Evaluation of the Effects of Donepezil, Memantine and alpha-lipoic Acid Combined Administration in Amnesia Rats on Impaired Cognitive Functions in Terms of Behavioural, Apoptotic, Cholinergic and Glutamatergic Systems", CLINICAL AND EXPERIMENTAL HEALTH SCIENCES, cilt.11, sa.4, ss.733-741, 2021