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Do platelet apoptosis, activation, aggregation, lipid peroxidation and platelet-leukocyte aggregate formation occur simultaneously in hyperlipidemia?

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dc.contributor.authorŞENER, AZİZE
dc.contributor.authorsSener, A; Ozsavci, D; Oba, R; Demirel, Y; Uras, F; Yardimci, KT
dc.date.accessioned2022-03-12T17:19:36Z
dc.date.available2022-03-12T17:19:36Z
dc.date.issued2005
dc.description.abstractObjectives: The circulating lipoproteins may cause some abnormalities in platelet composition and function in hypercholesterolemia. The aim Of this Study was to investigate whether platelet apoptosis, platelet activation, platelet aggregation, platelet-leukocyte aggregate (PLA) formation and lipid peroxidation occur simultaneously in hyperlipidemia. Design and methods: Expression of GpIIb/IIIa (CD41a), P-selectin (CD62-P), platelet-bound fibrinogen (antifibrinogen), platelet membrane phosphatidylserine (PS), platelet-monocyte aggregates (mono-PLA) and platelet-neutrophil aggregates (neut-PLA) was measured in eight hyperlipidemic and eight normal subjects using flow cytometry. ADP (10 mu M) was used to activate platelets. Furthermore, ADP induced platelet aggregation responses, platelet malondialdehyde (MDA) and glutathione (GSH) levels were determined. Results: Before platelet activation, platelet CD62-P, anti Fibrinogen, annexin-V, mono-PLA, neut-PLA and platelet MDA levels as well as platelet aggregation responses in the hyperlipidemics were significantly higher than those in the controls (P < 0.01, P < 0.01, P < 0.01, P < 0.001, P < 0.001, P < 0.01, P < 0.001, respectively), whereas GpIIb/IIIa expression and GSH levels were not different significantly (P > 0.05). In the control group, CD62-P, antifibrinogen and annexin-V levels increased significantly after ADP activation (P < 0.05, P < 0.05, P < 0.01, respectively). In hyperlipidemic Subjects, annexin-V expression increased significantly after activation (P < 0.01), whereas expression of GpIIb/ IIIa, CD62-P and anti fibrinogen remained unchanged (P > 0.05). The levels of total cholesterol (T-CHO), low density lipoprotein cholesterol (LDL-C), serum fibrinogen (S-FGN) and high density lipoprotein cholesterol (HDL-C) in patients were found to be correlated with platelet CD62-P, anti Fibrinogen, annexin-V, mono-PLA and MDA. Conclusions: In conclusion, it seems that in hyperlipidemia, some platelets are in an activated state in circulation, and that increased lipid peroxidation, early apoptosis, platelet-leukocytes aggregate formation and platelet aggregation altogether accompany this process. (C) 2005 The Canadian Society of Clinical Chemists. All rights reserved.
dc.identifier.doi10.1016/j.clinbiochem.2005.09.005
dc.identifier.eissn1873-2933
dc.identifier.issn0009-9120
dc.identifier.pubmed16253220
dc.identifier.urihttps://hdl.handle.net/11424/228131
dc.identifier.wosWOS:000234502900006
dc.language.isoeng
dc.publisherPERGAMON-ELSEVIER SCIENCE LTD
dc.relation.ispartofCLINICAL BIOCHEMISTRY
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjecthyperlipidemia
dc.subjectapoptosis
dc.subjectannexin-V
dc.subjectplatelet activation
dc.subjectplatelet-leukocyte aggregates
dc.subjectplatelet MDA
dc.subjectGSH
dc.subjectGpIIb/IIIa
dc.subjectP-selectin
dc.subjectFLOW-CYTOMETRY
dc.subjectFIBRINOGEN BINDING
dc.subjectWHOLE-BLOOD
dc.subjectP-SELECTIN
dc.subjectEXPRESSION
dc.subjectMICROPARTICLES
dc.subjectLIPOPROTEINS
dc.subjectSECRETION
dc.subjectVIVO
dc.titleDo platelet apoptosis, activation, aggregation, lipid peroxidation and platelet-leukocyte aggregate formation occur simultaneously in hyperlipidemia?
dc.typearticle
dspace.entity.typePublication
local.avesis.idbc7cd0cb-a5a4-4ef2-aa04-b6f53dac2848
local.import.packageSS17
local.indexed.atWOS
local.indexed.atSCOPUS
local.indexed.atPUBMED
local.journal.numberofpages7
oaire.citation.endPage1087
oaire.citation.issue12
oaire.citation.startPage1081
oaire.citation.titleCLINICAL BIOCHEMISTRY
oaire.citation.volume38
relation.isAuthorOfPublicationfd65174e-4126-41c3-913d-e8bcdce20632
relation.isAuthorOfPublication.latestForDiscoveryfd65174e-4126-41c3-913d-e8bcdce20632

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