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PIK3CA and TP53 MUTATIONS and SALL4, PTEN and PIK3R1 GENE EXPRESSION LEVELS in BREAST CANCER

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dc.contributor.authorKAYA, HANDAN
dc.contributor.authorsDirican, Ebubekir; Seven, Ipek Erbarut; Kaya, Handan; Ugurlu, M. Umit; Peker, Irem; Gulluoglu, Bahadir M.; Ozer, Ayse; Akkiprik, Mustafa
dc.date.accessioned2022-03-12T22:41:31Z
dc.date.available2022-03-12T22:41:31Z
dc.date.issued2020
dc.description.abstractObjective: A high frequency of PI3K signalling pathway abnormalities and TP53 mutations are critical in the development and progression of breast cancer (BCa). We aimed to detect PIK3CA and TP53 mutations via an expression analysis of PIK3R1, PTEN and SALL4 and correlate the expression of these genes with clinical parameters of BCa. Materials and methods: PIK3CA and TP53 mutations in BCa samples were analysed by High-Resolution Melting (HRM) analysis, followed by Sanger sequencing, and the expression levels of PIK3R1, PTEN and SALL4 were evaluated by RT-PCR methods. Results: The frequency of PIK3CA and TP53 mutations was 42% and 38% according to the HRM and Sanger sequencing. There was a significantly high frequency of these mutations in ER(+), N0 and HER2(-) tumour samples. PIK3R1 and PTEN expression levels were high in tumour samples, whereas SALL4 expression was low. In patients with TP53 mutations, PIK3R1 expression was low, and this finding was statistically significant. PIK3R1 and PTEN expression levels showed statistically significant, respectively in G3 grades, ER(+), (PR)(+), HER2(+) and ER(+). Conclusions: We suggest that these candidate genes could be potential prognostic biomarkers of BCa and that they should be considered in the evaluation of clinical parameters of BCa.
dc.identifier.doi10.1515/tjb-2019-0137
dc.identifier.eissn1303-829X
dc.identifier.issn0250-4685
dc.identifier.urihttps://hdl.handle.net/11424/236127
dc.identifier.wosWOS:000582568000007
dc.language.isoeng
dc.publisherWALTER DE GRUYTER GMBH
dc.relation.ispartofTURKISH JOURNAL OF BIOCHEMISTRY-TURK BIYOKIMYA DERGISI
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectBreast cancer
dc.subjectPI3K
dc.subjectPIK3CA
dc.subjectHRM
dc.subjectCandidate genes
dc.subjectCELL-PROLIFERATION
dc.subjectASSOCIATION
dc.titlePIK3CA and TP53 MUTATIONS and SALL4, PTEN and PIK3R1 GENE EXPRESSION LEVELS in BREAST CANCER
dc.typearticle
dspace.entity.typePublication
local.avesis.idb8b476e2-e766-4f4a-896b-e79645f6fcc4
local.import.packageSS17
local.indexed.atWOS
local.indexed.atTRDIZIN
local.journal.numberofpages9
local.journal.quartileQ4
oaire.citation.endPage523
oaire.citation.issue5
oaire.citation.startPage515
oaire.citation.titleTURKISH JOURNAL OF BIOCHEMISTRY-TURK BIYOKIMYA DERGISI
oaire.citation.volume45
relation.isAuthorOfPublication0bf301ff-2544-4a5e-b38b-b43084d6c255
relation.isAuthorOfPublication.latestForDiscovery0bf301ff-2544-4a5e-b38b-b43084d6c255

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