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Community changes during start-up in methanogenic bioreactors exposed to increasing levels of ammonia

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dc.contributor.authorÇALLI, BARIŞ
dc.contributor.authorsCalli, B; Mertoglu, B; Inanc, B; Yenigun, O
dc.date.accessioned2022-03-12T17:21:45Z
dc.date.available2022-03-12T17:21:45Z
dc.date.issued2005
dc.description.abstractTo investigate the methanogenic population dynamics during the start-up under gradually increasing free ammonia levels, five mesophilic UASB reactors seeded with different sludges were operated for 140 days. Changes in the methanogenic community investigated by using 16S rDNA/rRNA based molecular methods such as denaturing gradient gel electrophoresis (DGGE), fluorescent in-situ hybridization (FISH), cloning and DNA sequencing. Free ammonia nitrogen (FAN) in the reactors was gradually increased from 50 to 130 mg l(-1) by increasing total ammonia nitrogen (TAN) from 1000 to 2500 mg l(-1) in the feed and keeping reactor pH at 7.7 in the active zone. Even at highest FAN level, COD and VFA removal efficiencies above 90 and 98% were obtained, respectively. However, Methanosaeta-related species mainly prevailing in seed sludges were substituted for Methanosarcina as the abundant methanogens although reactor performances were almost comparable and constant.
dc.identifier.doi10.1080/09593332608618585
dc.identifier.eissn1479-487X
dc.identifier.issn0959-3330
dc.identifier.pubmed15747603
dc.identifier.urihttps://hdl.handle.net/11424/228362
dc.identifier.wosWOS:000227708300009
dc.language.isoeng
dc.publisherTAYLOR & FRANCIS LTD
dc.relation.ispartofENVIRONMENTAL TECHNOLOGY
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectDGGE
dc.subjectFISH
dc.subjectfree ammonia
dc.subjectMethanosaeta
dc.subjectMethanosarcina
dc.subjectANAEROBIC-DIGESTION
dc.subjectMOISTURE-CONTENT
dc.subjectSLUDGE
dc.subjectINHIBITION
dc.subjectDIVERSITY
dc.subjectLANDFILL
dc.subjectREACTORS
dc.subjectWASTE
dc.subjectPH
dc.titleCommunity changes during start-up in methanogenic bioreactors exposed to increasing levels of ammonia
dc.typearticle
dspace.entity.typePublication
local.avesis.idd33d586e-96cc-46e7-958e-63361ba81521
local.import.packageSS17
local.indexed.atWOS
local.indexed.atSCOPUS
local.indexed.atPUBMED
local.journal.numberofpages7
oaire.citation.endPage91
oaire.citation.issue1
oaire.citation.startPage85
oaire.citation.titleENVIRONMENTAL TECHNOLOGY
oaire.citation.volume26
relation.isAuthorOfPublication2e20fe96-469a-44e0-88b2-e56450a93cf6
relation.isAuthorOfPublication.latestForDiscovery2e20fe96-469a-44e0-88b2-e56450a93cf6

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