Publication:
Effects of different forms of chitosan on intercellular junctions of mouse fibroblasts in vitro

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dc.contributor.authorÖZBAŞ, SUNA
dc.contributor.authorsUslu, B.; Biltekin, B.; Denir, S.; Ozbas-Turan, S.; Arbak, S.; Akbuga, J.; Bilir, A.
dc.date.accessioned2022-03-12T20:30:04Z
dc.date.available2022-03-12T20:30:04Z
dc.date.issued2016
dc.description.abstractChitosan is a linear polysaccharide that has many biomedical applications. We compared the effects of chitosan, in both solution and membranous form, on intercellular adhesion of Swiss 3T3 mouse fibroblasts. Cells were grown as spheroidal cell cultures. Some control cell spheroids were cultured without chitosan and two experimental groups were cultured with chitosan. Chitosan in solution was used for one experimental group and chitosan in membranous form was used for the other. For each group, intercellular adhesion was investigated on days 5 and 10 of culture. Transmission electron microscopy revealed well-defined cellular projections that were more prominent in cells exposed to either membranous or solution forms of chitosan than to the chitosan-free control. Immunocytochemical staining of ICAM-1 and e-cadherin was used to determine the development of intercellular junctions. Compared to the weakly stained control, strong reactions were observed in both chitosan exposed groups at both 5 and 10 days. Cells were treated with 5-bromo-2-deoxyuridine (BrdU) and incubated with anti-BrdU primary antibody to assess proliferation. Both the solution and membranous forms of chitosan increased proliferation at both 5 and 10 days. Cellular viability was assessed using 3-(4,5-dimethylthiazol-2-yl)-2,5diphenyltetrazolium bromide (MTT). The MTT assay indicated high cell viability; maximum viability was obtained with the solution form of chitosan at day 5. Chitosan exposure increased the number of intercellular junctions and showed a significant proliferative effect on 3T3 mouse fibroblasts.
dc.identifier.doi10.3109/10520295.2015.1064998
dc.identifier.eissn1473-7760
dc.identifier.issn1052-0295
dc.identifier.pubmed26523482
dc.identifier.urihttps://hdl.handle.net/11424/234148
dc.identifier.wosWOS:000377636600003
dc.language.isoeng
dc.publisherTAYLOR & FRANCIS INC
dc.relation.ispartofBIOTECHNIC & HISTOCHEMISTRY
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectchitosan
dc.subjecte-cadherin
dc.subjectelectron microscopy
dc.subjectICAM-1
dc.subjectTISSUE ENGINEERING APPLICATIONS
dc.subjectGENE DELIVERY
dc.subjectCHITIN
dc.subjectHYDROGEL
dc.subjectPROLIFERATION
dc.subjectSCAFFOLDS
dc.subjectADHESION
dc.subjectGROWTH
dc.subjectDEGRADATION
dc.subjectMACROPHAGES
dc.titleEffects of different forms of chitosan on intercellular junctions of mouse fibroblasts in vitro
dc.typearticle
dspace.entity.typePublication
local.avesis.id5b84c1e4-2741-455e-bb4f-a462954fcd63
local.import.packageSS17
local.indexed.atWOS
local.indexed.atSCOPUS
local.indexed.atPUBMED
local.journal.numberofpages10
local.journal.quartileQ4
oaire.citation.endPage29
oaire.citation.issue1
oaire.citation.startPage20
oaire.citation.titleBIOTECHNIC & HISTOCHEMISTRY
oaire.citation.volume91
relation.isAuthorOfPublicationd56d5fb1-37ec-4be6-8156-b58d67614019
relation.isAuthorOfPublication.latestForDiscoveryd56d5fb1-37ec-4be6-8156-b58d67614019

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