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Determination of reactive oxygen species in myringotomized tympanic membranes: Effect of vitamin E treatment

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dc.contributor.authorHAKLAR, GONCAGÜL
dc.contributor.authorsPolat, S; Ozturk, O; Uneri, C; Yuksel, M; Haklar, G; Bozkurt, S; Kullu, S
dc.date.accessioned2022-03-12T17:16:39Z
dc.date.available2022-03-12T17:16:39Z
dc.date.issued2004
dc.description.abstractObjectives/Hypothesis: Recent studies have established a strong relationship between the development of myringosclerosis and reactive oxygen species (ROS). The aims of the present study were to directly detect ROS in the tympanic membrane and middle ear mucosa of rats by measuring luminol amplified chemiluminescence, to evaluate the changes in the levels of ROS after treatment with vitamin E, and to examine the possible changes in the tympanic membranes otomicroscopically and histologically. Study Design: Prospective controlled animal study. Methods: Forty healthy Sprague-Dawley rats were divided into five groups of eight animals each. Animals in all groups except group 1 were bilaterally myringotomized. Group 2 received no treatment, group 3 was treated with topical olive oil, group 4 received topical vitamin E, and group 5 received intramuscular vitamin E. After 24 hours of myringotomy, tympanic membranes were examined otomicroscopically; thereafter, tympanic membranes and middle ear mucosa were peeled off. The right ears of the animals were used for biochemical assay, and the left ears were used for histological study. Results: Reactive oxygen species levels were significantly decreased in group 4 with topical application of vitamin E compared with untreated and myringotomized animals in group 2. Reactive oxygen species levels were also decreased in group 5, although the decrease was not statistically significant when compared with groups 2 and 3. Histological studies confirmed sclerotic changes in the untreated myringotomized animals. The tympanic membranes of animals in groups 2 and 3 showed a white, chalk-like pattern of sclerotic changes, whereas animals in groups 4 and 5, with the exception of two animals in group 5, lacked these changes. Conclusion: Although the relationship between the development of myringosclerosis and ROS had been well documented previously, the present study is the first that has directly measured the levels of ROS in the tympanic membrane and middle ear mucosa. These results are relevant because they correlate with histological findings. It has also been demonstrated that topically applied vitamin E is effective in decreasing the ROS levels.
dc.identifier.doi10.1097/00005537-200404000-00023
dc.identifier.eissn1531-4995
dc.identifier.issn0023-852X
dc.identifier.pubmed15064630
dc.identifier.urihttps://hdl.handle.net/11424/227649
dc.identifier.wosWOS:000220806100022
dc.language.isoeng
dc.publisherWILEY
dc.relation.ispartofLARYNGOSCOPE
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectACUTE OTITIS-MEDIA
dc.subjectMIDDLE-EAR
dc.subjectGUINEA-PIGS
dc.subjectLIPID-PEROXIDATION
dc.subjectRADICAL INJURY
dc.subjectOLIVE OIL
dc.subjectMYRINGOSCLEROSIS
dc.subjectTYMPANOSCLEROSIS
dc.subjectCHEMILUMINESCENCE
dc.subjectANTIOXIDANTS
dc.titleDetermination of reactive oxygen species in myringotomized tympanic membranes: Effect of vitamin E treatment
dc.typearticle
dspace.entity.typePublication
local.avesis.id2cf57055-5bcc-4f6d-88e5-1eead939e5d3
local.import.packageSS17
local.indexed.atWOS
local.indexed.atSCOPUS
local.indexed.atPUBMED
local.journal.numberofpages6
oaire.citation.endPage725
oaire.citation.issue4
oaire.citation.startPage720
oaire.citation.titleLARYNGOSCOPE
oaire.citation.volume114
relation.isAuthorOfPublication27f9fd8a-7791-4b61-a4c5-518c52435a15
relation.isAuthorOfPublication.latestForDiscovery27f9fd8a-7791-4b61-a4c5-518c52435a15

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