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Utilization of orange peel waste for sustainable amino acid production by Corynebacterium glutamicum

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dc.contributor.authorSARIYAR AKBULUT, BERNA
dc.contributor.authorsJunker N., SARIYAR AKBULUT B., Wendisch V. F.
dc.date.accessioned2024-08-05T12:47:20Z
dc.date.available2024-08-05T12:47:20Z
dc.date.issued2024-01-01
dc.description.abstractOranges are the most processed fruit in the world–it is therefore apparent that the industrial production of orange juice generates large quantities of orange peel as a by-product. Unfortunately, the management of the orange peel waste leads to economic and environmental problems. Meanwhile, the use of sustainable raw materials for the production of bulk chemicals, such as amino acids, is becoming increasingly attractive. To address both issues, this study focused on the use of orange peel waste as a raw material for media preparation for the production of amino acids by engineered Corynebacterium glutamicum. C. glutamicum grew on pure orange peel hydrolysate (OPH) and growth was enhanced by the addition of a nitrogen source and a pH buffer. Inhibitory effects by the combination of high concentrations of OPH, (NH4)2SO4, and MOPS buffer in the wild-type strain (WT), were overcome in the tyrosine-producing engineered C. glutamicum strain AROM3. Genetic modifications that we identified to allow for improved growth rates under these conditions included the deletions of the vanillin dehydrogenase gene vdh, the ʟ-lactate dehydrogenase gene ldhA and the 19 genes comprising cluster cg2663-cg2686. A growth inhibiting compound present in high concentrations in the OPH is 5-(hydroxymethyl)furfural (HMF). We identified vdh as being primarily responsible for the oxidation of HMF to its acid 5-hydroxymethyl-2-furancarboxylic acid (HMFCA), as the formation of HMFCA was reduced by 97% upon deletion of vdh in C. glutamicum WT. In addition, we showed that growth limitations could be overcome by adjusting the media preparation, using a combination of cheap ammonia water and KOH for pH neutralization after acidic hydrolysis. Overall, we developed a sustainable medium based on orange peel waste for the cultivation of C. glutamicum and demonstrated the successful production of the exemplary amino acids ʟ-arginine, ʟ-lysine, ʟ-serine, ʟ-valine and ʟ-tyrosine.
dc.identifier.citationJunker N., SARIYAR AKBULUT B., Wendisch V. F., "Utilization of orange peel waste for sustainable amino acid production by Corynebacterium glutamicum", Frontiers in Bioengineering and Biotechnology, cilt.12, 2024
dc.identifier.doi10.3389/fbioe.2024.1419444
dc.identifier.issn2296-4185
dc.identifier.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85199364724&origin=inward
dc.identifier.urihttps://hdl.handle.net/11424/297393
dc.identifier.volume12
dc.language.isoeng
dc.relation.ispartofFrontiers in Bioengineering and Biotechnology
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectTıp
dc.subjectCerrahi Tıp Bilimleri
dc.subjectPatoloji
dc.subjectBiyomedikal Mühendisliği
dc.subjectYaşam Bilimleri
dc.subjectBiyoteknoloji
dc.subjectSağlık Bilimleri
dc.subjectTemel Tıp Bilimleri
dc.subjectBiyokimya
dc.subjectTemel Bilimler
dc.subjectMühendislik ve Teknoloji
dc.subjectMedicine
dc.subjectSurgery Medicine Sciences
dc.subjectPathology
dc.subjectBiomedical Engineering
dc.subjectLife Sciences
dc.subjectBiotechnology
dc.subjectHealth Sciences
dc.subjectFundamental Medical Sciences
dc.subjectBiochemistry
dc.subjectNatural Sciences
dc.subjectEngineering and Technology
dc.subjectMühendislik, Bilişim ve Teknoloji (ENG)
dc.subjectYaşam Bilimleri (LIFE)
dc.subjectMühendislik
dc.subjectBiyoloji ve Biyokimya
dc.subjectMikrobiyoloji
dc.subjectPATOLOJİ
dc.subjectBİYOTEKNOLOJİ VE UYGULAMALI MİKROBİYOLOJİ
dc.subjectMÜHENDİSLİK, BİYOMEDİKAL
dc.subjectEngineering, Computing & Technology (ENG)
dc.subjectLife Sciences (LIFE)
dc.subjectENGINEERING
dc.subjectBIOLOGY & BIOCHEMISTRY
dc.subjectMICROBIOLOGY
dc.subjectPATHOLOGY
dc.subjectBIOTECHNOLOGY & APPLIED MICROBIOLOGY
dc.subjectENGINEERING, BIOMEDICAL
dc.subjectBiyomühendislik
dc.subjectFizik Bilimleri
dc.subjectHistoloji
dc.subjectBiyomedikal mühendisliği
dc.subjectBioengineering
dc.subjectPhysical Sciences
dc.subjectHistology
dc.subjectagriculture sidestream
dc.subjectCorynebacterium glutamicum
dc.subjectfood waste upcycling
dc.subjectorange peel
dc.subjectsecond generation feed stock
dc.subjectsustainable amino acid production
dc.titleUtilization of orange peel waste for sustainable amino acid production by Corynebacterium glutamicum
dc.typearticle
dspace.entity.typePublication
local.avesis.id6e9b1e6d-0440-4182-b680-250f1d8a5849
local.indexed.atPUBMED
local.indexed.atSCOPUS
relation.isAuthorOfPublicationa9f127d3-8332-44dd-a532-34f3ef20bdb5
relation.isAuthorOfPublication.latestForDiscoverya9f127d3-8332-44dd-a532-34f3ef20bdb5

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