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Fabrication of ethosuximide loaded alginate/polyethylene oxide scaffolds for epilepsy research using 3D-printing method

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dc.contributor.authorULAĞ, SONGÜL
dc.contributor.authorGÜLHAN, REZZAN
dc.contributor.authorUS, ZEYNEP
dc.contributor.authorGÜNDÜZ, OĞUZHAN
dc.contributor.authorsKarabulut H., Dutta A., Moukbil Y., Cisen Akyol A., ULAĞ S., Aydin B., GÜLHAN R., US Z., Kalaskar D. M., GÜNDÜZ O.
dc.date.accessioned2023-12-25T08:52:51Z
dc.date.available2023-12-25T08:52:51Z
dc.date.issued2023-01-01
dc.description.abstractEpilepsy is a medical condition that causes seizures and impairs the mental and physical activities of patients. Unfortunately, over one-third of patients do not receive adequate relief from oral Antiepileptic Drugs (AEDs) and continue to experience seizures. In addition to that, long term usage of Antiepileptic Drugs can cause a range of side effects. To overcome this problem, the precision of 3D printing technology is combined with the controlled release capabilities of biodegradable polymers, allowing for tailored and localized AED delivery to specific seizure sites. As a result of this novel technique, therapeutic outcomes can be enhanced, side effects of AEDs are minimized, and patient-specific dosage forms can be created. This study focused on the use of ethosuximide, an antiepileptic drug, at different concentrations (10, 13, and 15 mg) loaded into 3D-printed sodium alginate and polyethylene oxide scaffolds. The scaffolds contained varying concentrations (0.25%, 0.50%, and 0.75% w/v) and had varying pores created by 3D patterning sizes from 159.86 ± 19.9 µm to 240.29 ± 10.7 µm to optimize the releasing system for an intracranial administration. The addition of PEO changed the Tg and Tm temperatures from 65°C to 69°C and from 262°C to 267°C, respectively. Cytotoxicity assays using the human neuroblastoma cell line (SH-SY5Y) showed that cell metabolic activity reached 130% after 168 h, allowing the cells to develop into mature neural cells. In vitro testing demonstrated sustained ethosuximide release lasting 2 hours despite crosslinking with 3% CaCl2. The workpaves the way for the use of ethosuximide -loaded scaffolds for treating epilepsy.
dc.identifier.citationKarabulut H., Dutta A., Moukbil Y., Cisen Akyol A., ULAĞ S., Aydin B., GÜLHAN R., US Z., Kalaskar D. M., GÜNDÜZ O., "Fabrication of ethosuximide loaded alginate/polyethylene oxide scaffolds for epilepsy research using 3D-printing method", Frontiers in Bioengineering and Biotechnology, cilt.11, 2023
dc.identifier.doi10.3389/fbioe.2023.1244323
dc.identifier.issn2296-4185
dc.identifier.urihttps://avesis.marmara.edu.tr/api/publication/b08b5d60-facf-4adc-86a2-6e311e252553/file
dc.identifier.urihttps://hdl.handle.net/11424/295969
dc.identifier.volume11
dc.language.isoeng
dc.relation.ispartofFrontiers in Bioengineering and Biotechnology
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectTıp
dc.subjectCerrahi Tıp Bilimleri
dc.subjectPatoloji
dc.subjectBiyomedikal Mühendisliği
dc.subjectYaşam Bilimleri
dc.subjectBiyoteknoloji
dc.subjectSağlık Bilimleri
dc.subjectTemel Tıp Bilimleri
dc.subjectBiyokimya
dc.subjectTemel Bilimler
dc.subjectMühendislik ve Teknoloji
dc.subjectMedicine
dc.subjectSurgery Medicine Sciences
dc.subjectPathology
dc.subjectBiomedical Engineering
dc.subjectLife Sciences
dc.subjectBiotechnology
dc.subjectHealth Sciences
dc.subjectFundamental Medical Sciences
dc.subjectBiochemistry
dc.subjectNatural Sciences
dc.subjectEngineering and Technology
dc.subjectMühendislik, Bilişim ve Teknoloji (ENG)
dc.subjectYaşam Bilimleri (LIFE)
dc.subjectMühendislik
dc.subjectBiyoloji ve Biyokimya
dc.subjectMikrobiyoloji
dc.subjectPATOLOJİ
dc.subjectBİYOTEKNOLOJİ VE UYGULAMALI MİKROBİYOLOJİ
dc.subjectMÜHENDİSLİK, BİYOMEDİKAL
dc.subjectEngineering, Computing & Technology (ENG)
dc.subjectLife Sciences (LIFE)
dc.subjectENGINEERING
dc.subjectBIOLOGY & BIOCHEMISTRY
dc.subjectMICROBIOLOGY
dc.subjectPATHOLOGY
dc.subjectBIOTECHNOLOGY & APPLIED MICROBIOLOGY
dc.subjectENGINEERING, BIOMEDICAL
dc.subjectBiyomühendislik
dc.subjectFizik Bilimleri
dc.subjectHistoloji
dc.subjectBiyomedikal mühendisliği
dc.subjectBioengineering
dc.subjectPhysical Sciences
dc.subjectHistology
dc.subject3D-printing
dc.subjectdrug resistant epilepsy
dc.subjectepilepsy treatment
dc.subjectimplantable scaffolds
dc.subjectpolyethylene oxide
dc.subjectsodium alginate
dc.titleFabrication of ethosuximide loaded alginate/polyethylene oxide scaffolds for epilepsy research using 3D-printing method
dc.typearticle
dspace.entity.typePublication
local.avesis.idb08b5d60-facf-4adc-86a2-6e311e252553
local.indexed.atPUBMED
local.indexed.atSCOPUS
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relation.isAuthorOfPublication.latestForDiscovery2c8f2a30-89dc-4a84-be40-116882ea2e16

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