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Optimizing the immobilization conditions of beta-galactosidase on UV-cured epoxy-based polymeric film using response surface methodology

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dc.contributor.authorOGAN, AYŞE
dc.contributor.authorKAHRAMAN, MEMET VEZİR
dc.contributor.authorDANIŞ, ÖZKAN
dc.contributor.authorDEMİR, SERAP
dc.contributor.authorsBeyler-Cigil, Asli; Danis, Ozkan; Sarsar, Onur; Kahraman, Memet Vezir; Ogan, Ayse; Demir, Serap
dc.date.accessioned2022-03-12T22:56:23Z
dc.date.available2022-03-12T22:56:23Z
dc.date.issued2021
dc.description.abstractUV-cured epoxy-based polymeric film was prepared from glycidyl methacrylate, trimethylolpropane triacrylate, and poly(ethylene glycol) methylether acrylate. 2-hydroxy-2- methylpropiophenone was used as photo initiator. Covalent binding through epoxy groups was employed to immobilize beta-galactosidase from Escherichia coli onto this film, and immobilization conditions were optimized by the response surface methodology. ATR-Fourier transform infrared (FTIR) and scanning electron microscopy (SEM) analysis was carried out to characterize the epoxy-based polymeric film. Immobilization yield of beta-galactosidase on the material was calculated as 3.57 mg/g and the highest enzyme activity for the immobilized enzyme recorded at pH 6.5 degrees C and 60 degrees C. The immobilized enzyme preserved 51% of its activity at the end of 12 runs. Free and immobilized enzyme hydrolyzed 163.8 and 172.3 mu M lactose from 1% lactose, respectively. Kinetic parameters of both free and immobilized beta-galactosidase were also investigated, and K-m values were determined to be 0.647 and 0.7263 mM, respectively. Practical applications In our study we prepared a UV-cured epoxy-based polymeric film and optimized the immobilization conditions of beta-galactosidase from Escherichia coli onto this polymeric film by using response surface methodology (RSM). For this purpose, three-level and three-factor Box-Behnken design, which is an independent, rotatable or nearly rotatable, quadratic design, was applied. Optimal levels of three variables, namely, the amount of enzyme, immobilization time, and pH were determined using Box-Behnken experimental design. Lactose hydrolysis studies were performed from milk and lactose samples using free and immobilized enzyme. In addition, kinetic parameters, storage stability, and re-usability of immobilized beta-galactosidase were examined.
dc.identifier.doi10.1111/jfbc.13699
dc.identifier.eissn1745-4514
dc.identifier.issn0145-8884
dc.identifier.pubmed33694174
dc.identifier.urihttps://hdl.handle.net/11424/236927
dc.identifier.wosWOS:000627263200001
dc.language.isoeng
dc.publisherWILEY
dc.relation.ispartofJOURNAL OF FOOD BIOCHEMISTRY
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectgalactosidase
dc.subjectenzyme immobilization
dc.subjectbased polymeric film
dc.subjectresponse surface methodology
dc.titleOptimizing the immobilization conditions of beta-galactosidase on UV-cured epoxy-based polymeric film using response surface methodology
dc.typearticle
dspace.entity.typePublication
local.avesis.idc0868c9b-98ff-44d3-bb71-db9b5db34624
local.import.packageSS17
local.indexed.atWOS
local.indexed.atSCOPUS
local.indexed.atPUBMED
local.journal.articlenumbere13699
local.journal.numberofpages11
oaire.citation.issue4
oaire.citation.titleJOURNAL OF FOOD BIOCHEMISTRY
oaire.citation.volume45
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relation.isAuthorOfPublication2790d85f-c14d-4e37-8567-e89f9e168058
relation.isAuthorOfPublication.latestForDiscovery58db3332-a5f8-4224-a021-aeea795f52fa

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