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A multifunctional natural treasure based on a “one stone, many birds” strategy for designing health-promoting applications: Tordylium apulum

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dc.contributor.authorEMRE, GİZEM
dc.contributor.authorsNilofar N., Zengin G., Uba A. I., Abul N., GÜLÇİN İ., Koyuncu I., Yuksekdag O., Kumar M Ponnaiya S., Tessappan S., Nazzaro F., et al.
dc.date.accessioned2024-09-24T11:15:23Z
dc.date.available2024-09-24T11:15:23Z
dc.date.issued2024-12-01
dc.description.abstractWild plants provide important bioactive compounds, and their analysis relies heavily on selecting the right extraction techniques and solvents. This study was conducted to determine the phenolic content and biopharmaceutical potential of four different extracts (ethyl acetate, ethanol, 70% ethanol, and water) from the aerial parts of wild plant Tordylium apulum L. The biochemical profile of the extract was screened using high performance liquid chromatography -mass spectrometry (HPLC-MS) analysis. The total phenolic and flavonoid content was examined using the Folin-Ciocalteu assay and the aluminium trichloride assay, respectively. The antioxidant activity was evaluated through several tests, including 2,2-Diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), cupric reducing antioxidant capacity (CUPRAC), ferric reducing antioxidant power (FRAP), phosphomolybdenum (PBD), and metal chelating activity (MCA). Five types of enzyme inhibition activity were tested against acetylcholinesterase (AChE), butrylcholinesterase (BChE), tyrosinase, α-amylase, and α-glucosidase. Additionally, For the first time, the inhibitory activity of T. apulum extract against human carbonic anhydrase isoenzymes I and II (hCA-I and hCA-II) was evaluated. Fifty-five compounds for negative ionization mode, and twenty-eight compounds for positive ionization mode were recorded in HPLC-MS analysis and they were polyphenolic, flavonoids, carbohydrates, sugar alcohol and amino acids. These results indicate that different solvents extract varying levels of antioxidants from T. apulum, with ethanol and water extracts generally exhibiting superior antioxidant activities. The ethanol extract of T. apulum exhibited the maximum contents of total phenolics measuring 33.71 mg gallic acid equivalent (GAE)/g. The ethanol extract exhibited the highest inhibition of AChE with 2.28 mg galanthamine equivalent (GALAE)/g. The ethyl acetate and ethanol extracts also showed the highest hCA-I and hCA-II inhibition potential, respectively. The ethanol-water and water extracts acted on the biofilm of E. coli (49.93% and 45.22%, respectively), and the biofilm of P. aeruginosa (50.68% and 44.46%, respectively). The extracts were tested on different cell lines for cytotoxic potentials and in particular the water extract induced the apoptotic pathways in cervical cancer (HELA) cell lines. In conclusion, T. apulum exhibit multidirectional biological properties and it could be considered as a versatile agent for the development of health-promoting applications.
dc.identifier.citationNilofar N., Zengin G., Uba A. I., Abul N., GÜLÇİN İ., Koyuncu I., Yuksekdag O., Kumar M Ponnaiya S., Tessappan S., Nazzaro F., et al., "A multifunctional natural treasure based on a “one stone, many birds” strategy for designing health-promoting applications: Tordylium apulum", Food Bioscience, cilt.62, 2024
dc.identifier.doi10.1016/j.fbio.2024.105088
dc.identifier.issn2212-4292
dc.identifier.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85203649864&origin=inward
dc.identifier.urihttps://hdl.handle.net/11424/297837
dc.identifier.volume62
dc.language.isoeng
dc.relation.ispartofFood Bioscience
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectTarımsal Bilimler
dc.subjectZiraat
dc.subjectGıda Mühendisliği
dc.subjectYaşam Bilimleri
dc.subjectMoleküler Biyoloji ve Genetik
dc.subjectSitogenetik
dc.subjectTemel Bilimler
dc.subjectMühendislik ve Teknoloji
dc.subjectAgricultural Sciences
dc.subjectAgriculture
dc.subjectFood Engineering
dc.subjectLife Sciences
dc.subjectMolecular Biology and Genetics
dc.subjectCytogenetic
dc.subjectNatural Sciences
dc.subjectEngineering and Technology
dc.subjectTarım Ve Çevre Bilimleri (Age)
dc.subjectYaşam Bilimleri (Life)
dc.subjectTarım Bilimleri
dc.subjectMoleküler Biyoloji Ve Genetik
dc.subjectGıda Bilimi Ve Teknolojisi
dc.subjectBiyokimya Ve Moleküler Biyoloji
dc.subjectAgriculture & Environment Sciences (Age)
dc.subjectLife Sciences (Life)
dc.subjectMolecular Biology & Genetics
dc.subjectFood Science & Technology
dc.subjectBiochemistry & Molecular Biology
dc.subjectYemek bilimi
dc.subjectBiyokimya
dc.subjectFood Science
dc.subjectBiochemistry
dc.subjectAnti-biofilm
dc.subjectApoptosis
dc.subjectCarbonic anhydrase
dc.subjectDiabetes mellitus
dc.subjectTordylium apulum
dc.subjectVersatile agents
dc.titleA multifunctional natural treasure based on a “one stone, many birds” strategy for designing health-promoting applications: Tordylium apulum
dc.typearticle
dspace.entity.typePublication
local.avesis.idd07b3fe9-62bf-4a0c-83a4-34f7cc6c9bb3
local.indexed.atSCOPUS
relation.isAuthorOfPublication056ad19f-5117-4589-98e2-80d0469dc104
relation.isAuthorOfPublication.latestForDiscovery056ad19f-5117-4589-98e2-80d0469dc104

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