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Treatment with chitin microparticles is protective against lung histopathology in a murine asthma model

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dc.contributor.authorAKKOÇ, TUNÇ
dc.contributor.authorsOzdemir, C.; Yazi, D.; Aydogan, M.; Akkoc, T.; Bahceciler, N. N.; Strong, P.; Barlan, I. B.
dc.date.accessioned2022-03-12T17:20:28Z
dc.date.available2022-03-12T17:20:28Z
dc.date.issued2006
dc.description.abstractChitin, a natural polysaccharide extracted from shrimp, is a potent T and B cell adjuvant when delivered in the form of chitin microparticles and can shift a polarized T-helper type 2 (Th2) immune response towards a Th1 response. We investigated the beneficial effects of the intranasal application of chitin microparticles in newborn mice before and after the establishment of a model of allergic asthma. Mice were grouped as asthma (A), primary prevention (PP), treatment (T), primary prevention+treatment (PPT) and control (C) groups. All mice except controls were sensitized with ovalbumin intraperitoneally and challenged intratracheally to establish the asthma model. Mice in the PP and PPT groups received chitin microparticles intranasally during the newborn period before sensitization. Mice in the PPT and T groups received intranasal chitin microparticles after challenge. Airway histopathology was evaluated in all groups. All of the airway histopathologic parameters of small and medium-sized airways of the T and PPT groups were significantly ameliorated when compared with the asthma model group. In the large airways, thicknesses of basement membrane, epithelium and subepithelial smooth muscle layers of the PPT group and basement membrane thicknesses of the T group were also significantly lower compared with the asthma model group. Comparison of the PP group with the asthma model group revealed significantly reduced goblet cell numbers and significantly reduced epithelial and basement membrane thicknesses in small and medium airways, in addition to significantly reduced basement membrane thicknesses in the medium-sized airways. Intranasal application of microgram quantities of chitin microparticles had a beneficial effect in preventing and treating histopathologic changes in the airways of asthmatic mice.
dc.identifier.doi10.1111/j.1365-2222.2006.02515.x
dc.identifier.eissn1365-2222
dc.identifier.issn0954-7894
dc.identifier.pubmed16839412
dc.identifier.urihttps://hdl.handle.net/11424/228239
dc.identifier.wosWOS:000238579700015
dc.language.isoeng
dc.publisherWILEY
dc.relation.ispartofCLINICAL AND EXPERIMENTAL ALLERGY
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectairway histopathology
dc.subjectasthma
dc.subjectBALB/c
dc.subjectchitin microparticles
dc.subjectACIDIC MAMMALIAN CHITINASE
dc.subjectHEAT-KILLED LISTERIA
dc.subjectT-CELL CYTOKINES
dc.subjectCPG OLIGODEOXYNUCLEOTIDES
dc.subjectATOPIC ASTHMA
dc.subjectINFLAMMATION
dc.subjectMODULATION
dc.subjectRESPONSES
dc.subjectADJUVANT
dc.subjectIMMUNITY
dc.titleTreatment with chitin microparticles is protective against lung histopathology in a murine asthma model
dc.typearticle
dspace.entity.typePublication
local.avesis.id3c2acbf9-4792-46df-89b2-36f799149fae
local.import.packageSS17
local.indexed.atWOS
local.indexed.atSCOPUS
local.indexed.atPUBMED
local.journal.numberofpages9
oaire.citation.endPage968
oaire.citation.issue7
oaire.citation.startPage960
oaire.citation.titleCLINICAL AND EXPERIMENTAL ALLERGY
oaire.citation.volume36
relation.isAuthorOfPublicationdde3c531-d82d-492a-96eb-d6724483eaa1
relation.isAuthorOfPublication.latestForDiscoverydde3c531-d82d-492a-96eb-d6724483eaa1

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