Publication:
Effects of carbachol on apoptosis in human chronic myelogenous leukemic K562 cell line

Simple item page
dc.contributor.authorAYDIN OMAY, BANU
dc.contributor.authorsAydin, Banu; Tulunay, Aysin; Eksioglu-Demiralp, Emel; Kan, Beki; Cabadak, Hulya
dc.date.accessioned2022-03-14T09:14:46Z
dc.date.available2022-03-14T09:14:46Z
dc.date.issued2019-01-31
dc.description.abstractObjectives: Muscarinic receptors mediate diverse actions of acetylcholine in the central nervous system and in non-nervous tissues innervated by the parasympathetic nervous system. Our study aims to evaluate the potential association of the M-3 muscarinic receptor with K562 cell proliferation and death. Materials and Methods: Cell proliferation was evaluated by bromodeoxyuridine (BrDU) incorporation. To show early, late apoptosis and cell death, cells were labelled with Annexin V, propidium iodide (PI) and analyzed by flow cytometry. Nuclear extracellular signal-regulated kinase (ERK/pERK) expression was measured by western blot analysis. Results: Treatment with carbachol (CCh) for 48h decreased cell number. Exposing K562 cells to CCh for 24h decreased the number of early apoptotic cells but did not change the number of late apoptotic and necrotic cells. CCh treatment for 48h increased the number of necrotic cells, but decreased the number of early and late apoptotic cells. In response to CCh, nuclear ERK expression was increased and this effect was reversed by 1,1-dimethyl-4-diphenylacetoxypiperidinium iodide (4DAMP). Nuclear pERK expression was decreased in CCh treated cells, 4DAMP did not reverse the effect. Conclusion: Our data suggest that cholinergic agonist CCh affects cell proliferation in K562 cells not only through muscarinic receptors but also through other cholinergic receptors.
dc.identifier.doi10.5472/marumj.518983
dc.identifier.issn1309-9469
dc.identifier.urihttps://hdl.handle.net/11424/242843
dc.identifier.wosWOS:000457122100007
dc.language.isoeng
dc.publisherMARMARA UNIV, FAC MEDICINE
dc.relation.ispartofMARMARA MEDICAL JOURNAL
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectMuscarinic receptors
dc.subjectK562 cells
dc.subjectCarbachol
dc.subjectCholinergic system
dc.subjectMUSCARINIC RECEPTORS
dc.subjectCANCER CELLS
dc.subjectGROWTH
dc.subjectPROLIFERATION
dc.subjectACETYLCHOLINE
dc.subjectPATHWAYS
dc.subjectNICOTINE
dc.subjectABLATION
dc.subjectDEATH
dc.subjectM-3
dc.titleEffects of carbachol on apoptosis in human chronic myelogenous leukemic K562 cell line
dc.typearticle
dspace.entity.typePublication
local.avesis.idf441f3ce-b8cf-4585-8a97-46e629575b48
local.import.packageSS16
local.indexed.atWOS
local.indexed.atSCOPUS
local.indexed.atTRDIZIN
local.journal.numberofpages6
oaire.citation.endPage43
oaire.citation.issue1
oaire.citation.startPage38
oaire.citation.titleMARMARA MEDICAL JOURNAL
oaire.citation.volume32
relation.isAuthorOfPublicationaf9e48cc-cef6-4af2-bdb7-5b424aadfc2b
relation.isAuthorOfPublication.latestForDiscoveryaf9e48cc-cef6-4af2-bdb7-5b424aadfc2b

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Aydin et al. - 2019 - Effects of carbachol on apoptosis in human chronic.pdf
Size:
443.13 KB
Format:
Adobe Portable Document Format
Download

Collections

Research Outputs