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Immobilization of alpha- amylase on aminated polyimide membrane: Preparation, characterization, and properties

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dc.contributor.authorÇAKMAKÇI, EMRAH
dc.contributor.authorsCakmakci, Emrah; Cigil, Asli Beyler; Danis, Ozkan; Demir, Serap; Kahraman, Memet Vezir
dc.date.accessioned2022-03-13T12:44:38Z
dc.date.available2022-03-13T12:44:38Z
dc.date.issued2014
dc.description.abstract-amylase was covalently immobilized on functionalized polyimide (PI) membranes via glutaraldehyde (GA) activation. 3,3,4,4-Benzophenonetetracarboxylic acid dianhydride (BTDA) and 4,4-oxydianline (4,4-ODA) based polyimide membranes were obtained via thermal imidization. Free amine groups on the surface of the polyimide membranes were generated by the amination reaction of polyimides with hexamethylenediamine (HMDA). Surface-aminated membranes were subjected to enzyme immobilization after GA activation. Immobilization efficiency and enzyme activity of -amylase was examined at various pH (3.0-8.0) and temperature (15-80 degrees C). The storage stability and reusability of immobilized -amylase were investigated. Immobilization yield was found as 359.53mg per gram of modified polyimide films. Enzyme assays demonstrated that the immobilized enzyme exhibited better thermo stability than the free one. The storage stability and reusability improved by the immobilization on this enzyme support. Free enzyme lost its activity completely within 15 days. On the other hand, the immobilized enzyme retained 79.98% of its activity after 30 days. These results confirmed that -amylase was successfully immobilized and gained more stable character compared to the free enzyme.
dc.identifier.doi10.1002/star.201300160
dc.identifier.eissn1521-379X
dc.identifier.issn0038-9056
dc.identifier.urihttps://hdl.handle.net/11424/237600
dc.identifier.wosWOS:000332335900006
dc.language.isoeng
dc.publisherWILEY-V C H VERLAG GMBH
dc.relation.ispartofSTARCH-STARKE
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectAlpha amylase
dc.subjectBTDA-ODA
dc.subjectEnzyme immobilization
dc.subjectPolyimide
dc.subjectSurface amination
dc.subjectSTARCH-CONVERTING ENZYMES
dc.subjectCOVALENT IMMOBILIZATION
dc.subjectMICROSPHERES
dc.subjectPERFORMANCE
dc.subjectSURFACE
dc.subjectBEADS
dc.titleImmobilization of alpha- amylase on aminated polyimide membrane: Preparation, characterization, and properties
dc.typearticle
dspace.entity.typePublication
local.avesis.ide05ab002-a560-4f93-a9ea-a2f8b22ac5fe
local.import.packageSS17
local.indexed.atWOS
local.indexed.atSCOPUS
local.journal.numberofpages7
oaire.citation.endPage280
oaire.citation.issue3-4
oaire.citation.startPage274
oaire.citation.titleSTARCH-STARKE
oaire.citation.volume66
relation.isAuthorOfPublication332869cd-e8a9-40ac-b678-cf5b01c6a6dc
relation.isAuthorOfPublication.latestForDiscovery332869cd-e8a9-40ac-b678-cf5b01c6a6dc

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