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Effect of extremely low frequency electromagnetic fields on bacterial membrane

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dc.contributor.authorGARİP, GÜNSELİ AYŞE
dc.contributor.authorsOncul, Sule; Cuce, Esra M.; Aksu, Burak; Garip, Ayse Inhan
dc.date.accessioned2022-03-12T20:28:31Z
dc.date.available2022-03-12T20:28:31Z
dc.date.issued2016
dc.description.abstractPurpose: The effect of extremely low frequency electromagnetic fields (ELF-EMF) on bacteria has attracted attention due to its potential for beneficial uses. This research aimed to determine the effect of ELF-EMF on bacterial membrane namely the membrane potential, surface potential, hydrophobicity, respiratory activity and growth.Materials and methods: Gram-positive Staphylococcus aureus and Gram-negative Escherichia coli were subjected to ELF-EMF, 50Hz, 1mT for 2h. Membrane potential was determined by fluorescence spectroscopy with or without EDTA (Ethylenediaminetetraacetic acid) with DisC3(5) (3,3-dipropylthiacarbocyanine iodide), zeta potential measurements were performed by electrophoretic mobility, hydrophobicity of the membrane was measured with MATH (Microbial Adhesion to Hydrocarbons) test, respiratory activity was determined with CTC (5-Cyano-2,3-ditolyl tetrazolium chloride), colony forming unit (CFU) and DAPI (4,6-diamidino-2-phenylindole, dihydrochloride) was used for growth determinations.Results: ELF-EMF caused changes in physicochemical properties of both Gram-positive and Gram-negative bacteria. Hyperpolarization was seen in S. aureus and EDTA-treated E. coli. Surface potential showed a positive shift in S. aureus contrariwise to the negative shift seen in EDTA-untreated E. coli. Respiratory activity increased in both bacteria. A slight decrease in growth was observed.Conclusion: These results show that ELF-EMF affects the crucial physicochemical processes in both Gram-positive and Gram-negative bacteria which need further research.
dc.identifier.doi10.3109/09553002.2015.1101500
dc.identifier.eissn1362-3095
dc.identifier.issn0955-3002
dc.identifier.pubmed26514970
dc.identifier.urihttps://hdl.handle.net/11424/233929
dc.identifier.wosWOS:000369276000005
dc.language.isoeng
dc.publisherTAYLOR & FRANCIS LTD
dc.relation.ispartofINTERNATIONAL JOURNAL OF RADIATION BIOLOGY
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectELF-EMF
dc.subjectbacterial membrane potential
dc.subjectsurface potential
dc.subjectbacterial hydrophobicity
dc.subjectrespiratory activity
dc.subjectbacterial growth
dc.subjectESCHERICHIA-COLI-CELLS
dc.subjectELF MAGNETIC-FIELDS
dc.subjectBIOSYNTHETIC REQUIREMENTS
dc.subjectELECTRIC-FIELDS
dc.subjectEXPOSURE
dc.subjectSURFACE
dc.subjectINHIBITION
dc.subjectREPAIR
dc.subjectDAMAGE
dc.subjectGROEL
dc.titleEffect of extremely low frequency electromagnetic fields on bacterial membrane
dc.typearticle
dspace.entity.typePublication
local.avesis.idc4a8b264-8d57-4793-9242-821b0be0c637
local.import.packageSS17
local.indexed.atWOS
local.indexed.atSCOPUS
local.indexed.atPUBMED
local.journal.numberofpages8
local.journal.quartileQ1
oaire.citation.endPage49
oaire.citation.issue1
oaire.citation.startPage42
oaire.citation.titleINTERNATIONAL JOURNAL OF RADIATION BIOLOGY
oaire.citation.volume92
relation.isAuthorOfPublication4e6e5b70-56a5-4519-bbe7-fde9a6ec4775
relation.isAuthorOfPublication.latestForDiscovery4e6e5b70-56a5-4519-bbe7-fde9a6ec4775

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