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Assessment of Berberine as a Multi-target Antimicrobial: A Multi-omics Study for Drug Discovery and Repositioning

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dc.contributor.authorSARIYAR AKBULUT, BERNA
dc.contributor.authorsKaraosmanoglu, Kubra; Sayar, Nihat Alpagu; Kurnaz, Isil Aksan; Akbulut, Berna Sariyar
dc.date.accessioned2022-03-13T12:45:32Z
dc.date.available2022-03-13T12:45:32Z
dc.date.issued2014
dc.description.abstractPostgenomics drug development is undergoing major transformation in the age of multi-omics studies and drug repositioning. Rather than applications solely in personalized medicine, omics science thus additionally offers a better understanding of a broader range of drug targets and drug repositioning. Berberine is an isoquinoline alkaloid found in many medicinal plants. We report here a whole genome microarray study in tandem with proteomics techniques for mining the plethora of targets that are putatively involved in the antimicrobial activity of berberine against Escherichia coli. We found DNA replication/repair and transcription to be triggered by berberine, indicating that nucleic acids, in general, are among its targets. Our combined transcriptomics and proteomics multi-omics findings underscore that, in the presence of berberine, cell wall or cell membrane transport and motility-related functions are also specifically regulated. We further report a general decline in metabolism, as seen by repression of genes in carbohydrate and amino acid metabolism, energy production, and conversion. An involvement of multidrug efflux pumps, as well as reduced membrane permeability for developing resistance against berberine in E. coli was noted. Collectively, these findings offer original and significant leads for omics-guided drug discovery and future repositioning approaches in the postgenomics era, using berberine as a multi-omics case study.
dc.identifier.doi10.1089/omi.2013.0100
dc.identifier.eissn1557-8100
dc.identifier.issn1536-2310
dc.identifier.pubmed24237354
dc.identifier.urihttps://hdl.handle.net/11424/237799
dc.identifier.wosWOS:000331085100005
dc.language.isoeng
dc.publisherMARY ANN LIEBERT, INC
dc.relation.ispartofOMICS-A JOURNAL OF INTEGRATIVE BIOLOGY
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectN-ACETYLTRANSFERASE ACTIVITY
dc.subjectCELL-DIVISION PROTEIN
dc.subjectESCHERICHIA-COLI
dc.subjectGENE-EXPRESSION
dc.subjectADDUCT FORMATION
dc.subjectEFFLUX PUMPS
dc.subjectRESISTANCE
dc.subjectBINDING
dc.subjectMEMBRANE
dc.subjectFTSZ
dc.titleAssessment of Berberine as a Multi-target Antimicrobial: A Multi-omics Study for Drug Discovery and Repositioning
dc.typearticle
dspace.entity.typePublication
local.avesis.idded13028-3204-41cf-b591-d847f1561ac7
local.import.packageSS17
local.indexed.atWOS
local.indexed.atSCOPUS
local.indexed.atPUBMED
local.journal.numberofpages12
oaire.citation.endPage53
oaire.citation.issue1
oaire.citation.startPage42
oaire.citation.titleOMICS-A JOURNAL OF INTEGRATIVE BIOLOGY
oaire.citation.volume18
relation.isAuthorOfPublicationa9f127d3-8332-44dd-a532-34f3ef20bdb5
relation.isAuthorOfPublication.latestForDiscoverya9f127d3-8332-44dd-a532-34f3ef20bdb5

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