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Response of Escherichia coli to Prolonged Berberine Exposure

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dc.contributor.authorSARIYAR AKBULUT, BERNA
dc.contributor.authorsGokgoz, Nilay Budeyri; Avci, Fatma Gizem; Yoneten, Kubra Karaosmanoglu; Alaybeyoglu, Begum; Ozkirimli, Elif; Sayar, Nihat Alpagu; Kazan, Dilek; Akbulut, Berna Sariyar
dc.date.accessioned2022-03-12T22:23:45Z
dc.date.available2022-03-12T22:23:45Z
dc.date.issued2017
dc.description.abstractBerberine is a plant-derived alkaloid possessing antimicrobial activity; unfortunately, its efflux through multidrug resistance pumps reduces its efficacy. Cellular life span of Escherichia coli is generally shorter with prolonged berberine exposure; nevertheless, about 30% of the cells still remain robust following this treatment. To elucidate its mechanism of action and to identify proteins that could be involved in development of antimicrobial resistance, protein profiles of E. coli cells treated with berberine for 4.5 and 8 hours were compared with control cells. A total of 42 proteins were differentially expressed in cells treated with berberine for 8 hours when compared to control cells. In both 4.5 and 8 hours of berberine-treated cells, carbohydrate and peptide uptake regimens remained unchanged, although amino acid maintenance regimen switched from transport to synthesis. Defect in cell division persisted and this condition was confirmed by images obtained from scanning electron microscopy. Universal stress proteins were not involved in stress response. The significant increase in the abundance of elongation factors could suggest the involvement of these proteins in protection by exhibiting chaperone activities. Furthermore, the involvement of the outer membrane protein OmpW could receive special attention as a protein involved in response to antimicrobial agents, since the expression of only this porin protein was upregulated after 8 hours of exposure.
dc.identifier.doi10.1089/mdr.2016.0063
dc.identifier.eissn1931-8448
dc.identifier.issn1076-6294
dc.identifier.pubmed27854150
dc.identifier.urihttps://hdl.handle.net/11424/234549
dc.identifier.wosWOS:000404988200001
dc.language.isoeng
dc.publisherMARY ANN LIEBERT, INC
dc.relation.ispartofMICROBIAL DRUG RESISTANCE
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectantimicrobial resistance
dc.subjectberberine
dc.subjectE
dc.subjectcoli
dc.subjectprotein map
dc.subjectstress response
dc.subjectOUTER-MEMBRANE PROTEINS
dc.subjectUNIVERSAL STRESS-PROTEIN
dc.subjectISOQUINOLINE ALKALOID BERBERINE
dc.subjectN-ACETYLTRANSFERASE ACTIVITY
dc.subjectFUNCTIONAL-CHARACTERIZATION
dc.subjectANTIBACTERIAL ACTIVITY
dc.subjectCHAPERONE PROPERTIES
dc.subjectACID RESISTANCE
dc.subjectGENE-EXPRESSION
dc.subjectIDENTIFICATION
dc.titleResponse of Escherichia coli to Prolonged Berberine Exposure
dc.typearticle
dspace.entity.typePublication
local.avesis.id105a2728-3c87-4eca-b134-1e129db0832d
local.import.packageSS17
local.indexed.atWOS
local.indexed.atSCOPUS
local.indexed.atPUBMED
local.journal.numberofpages14
local.journal.quartileQ3
oaire.citation.endPage544
oaire.citation.issue5
oaire.citation.startPage531
oaire.citation.titleMICROBIAL DRUG RESISTANCE
oaire.citation.volume23
relation.isAuthorOfPublicationa9f127d3-8332-44dd-a532-34f3ef20bdb5
relation.isAuthorOfPublication.latestForDiscoverya9f127d3-8332-44dd-a532-34f3ef20bdb5

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