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IL-1 beta is induced in reactive astrocytes in the somatosensory cortex of rats with genetic absence epilepsy at the onset of spike-and-wave discharges, and contributes to their occurrence

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dc.contributor.authorONAT, FİLİZ
dc.contributor.authorsAkin, Demet; Ravizza, Teresa; Maroso, Mattia; Carcak, Nihan; Eryigit, Tugba; Vanzulli, Ilaria; Aker, Rezzan Gulhan; Vezzani, Annamaria; Onat, Filiz Yilmaz
dc.date.accessioned2022-03-12T18:04:58Z
dc.date.available2022-03-12T18:04:58Z
dc.date.issued2011
dc.description.abstractInterleukin (IL)-1 beta plays a crucial role in the mechanisms of limbic seizures in rodent models of temporal lobe epilepsy. We addressed whether activation of the IL-1 beta signaling occurs in rats with genetic absence epilepsy (GAERS) during the development of spike-and-wave discharges (SWDs). Moreover, we studied whether inhibition of IL-1 beta biosynthesis in GAERS could affect SWD activity. IL-1 beta expression and glia activation were studied by immunocytochemistry in the forebrain of GAERS at postnatal days (PN)14, PN20, and PN90 and in age-matched non-epileptic control Wistar rats. In PN14 GAERS, when no SWDs have developed yet. UT immunostaining was undetectable, and astrocytes and microglia showed a resting phenotype similar to control Wistar rats. In 3 out of 9 PN20 GAERS, IL-1 beta was observed in activated astrocytes of the somatosensory cortex; the cytokine expression was associated with the occurrence of immature-type of SWDs. In all adult PN90 GAERS, when mature SWDs are established, IL-1 beta was observed in reactive astrocytes of the somatosensory cortex but not in adjacent cortical areas or in extra-cortical regions. An age-dependent c-fos activation was found in the somatosensory cortex of GAERS with maximal levels reached in PN90 rats; c-fos was also induced in some thalamic nuclei in PN20 and PN90 GAERS. Inhibition of IL-1 beta biosynthesis in PN90 GAERS by 4-day systemic administration of a specific ICE/Caspase-1 blocker, significantly reduced both SWD number and duration. These results show that IL-1 beta is induced in reactive astrocytes of the somatosensory cortex of GAERS at the onset of SWDs. IL-1 beta has pro-ictogenic properties in this model, and thus it may play a contributing role in the mechanisms underlying the occurrence of absence seizures. (C) 2011 Elsevier Inc. All rights reserved.
dc.identifier.doi10.1016/j.nbd.2011.05.015
dc.identifier.eissn1095-953X
dc.identifier.issn0969-9961
dc.identifier.pubmed21645619
dc.identifier.urihttps://hdl.handle.net/11424/230545
dc.identifier.wosWOS:000295816200001
dc.language.isoeng
dc.publisherACADEMIC PRESS INC ELSEVIER SCIENCE
dc.relation.ispartofNEUROBIOLOGY OF DISEASE
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectInflammation
dc.subjectAbsence seizures
dc.subjectInterleukins
dc.subjectSWD
dc.subjectEXPERIMENTAL FEBRILE SEIZURES
dc.subjectSTATUS EPILEPTICUS
dc.subjectGLUTAMATE UPTAKE
dc.subjectEPILEPTOGENESIS
dc.subjectEXPRESSION
dc.subjectMODEL
dc.subjectACTIVATION
dc.subjectINTERLEUKIN-1-BETA
dc.subjectHIPPOCAMPUS
dc.subjectCYTOKINES
dc.titleIL-1 beta is induced in reactive astrocytes in the somatosensory cortex of rats with genetic absence epilepsy at the onset of spike-and-wave discharges, and contributes to their occurrence
dc.typearticle
dspace.entity.typePublication
local.avesis.id88ee8960-a674-4e58-8c92-e667db874166
local.import.packageSS17
local.indexed.atWOS
local.indexed.atSCOPUS
local.journal.numberofpages11
oaire.citation.endPage269
oaire.citation.issue3
oaire.citation.startPage259
oaire.citation.titleNEUROBIOLOGY OF DISEASE
oaire.citation.volume44
relation.isAuthorOfPublicationc359dea3-046f-4397-90d5-62e4bfc31869
relation.isAuthorOfPublication.latestForDiscoveryc359dea3-046f-4397-90d5-62e4bfc31869

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