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3D printed artificial cornea for corneal stromal transplantation

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dc.contributor.authorŞAHİN, ALİ
dc.contributor.authorsUlag, Songul; Ilhan, Elif; Sahin, Ali; Yilmaz, Betul Karademir; Kalaskar, Deepak M.; Ekren, Nazmi; Kilic, Osman; Oktar, Faik Nuzhet; Gunduz, Oguzhan
dc.date.accessioned2022-03-14T09:22:35Z
dc.date.available2022-03-14T09:22:35Z
dc.date.issued2020-06
dc.description.abstractThe aim of this study is to understand the optical, biocompatible, and mechanical properties of chitosan (CS) and polyvinyl-alcohol (PVA) based corneal stroma constructs using 3D printing process. Corneal stroma is tested for biocompatibility with human adipose tissue-derived mesenchymal stem cells (hASCs). Physico-chemical and chemical characterization of the construct was performed using scanning electron microscopy (SEM), fourier transforms infrared spectroscopy (FTIR). Optical transmittance was analyzed using UV-Spectrophotometer. Results showed fabricated constructs have required shape and size. SEM images showed construct has thickness of 400 mu m. The FTIR spectra demonstrated the presence of various predicted peaks. The swelling and degradation studies of 13%(wt)PVA and 13%(wt)PVA/(1, 3, 5)%(wt)CS showed to have high swelling ratios of 7 days and degradation times of 30 days, respectively. The light transmittance values of the fabricated cornea constructs decreased with CS addition slightly. Tensile strength values decreased with increasing CS ratio, but we found to support intraocular pressure (IOP) which ranges from 12 to 22 mm-Hg. Preliminary biostability studies showed that composite constructs were compatible with hASCs even after 30 days' of degradation, showing potential for these cells to be differentiated to stroma layer in future. This study has implications for the rapid and custom fabrication of various cornea constructs for clinical applications.
dc.identifier.doi10.1016/j.eurpolymj.2020.109744
dc.identifier.eissn1873-1945
dc.identifier.issn0014-3057
dc.identifier.urihttps://hdl.handle.net/11424/243040
dc.identifier.wosWOS:000549179400001
dc.language.isoeng
dc.publisherPERGAMON-ELSEVIER SCIENCE LTD
dc.relation.ispartofEUROPEAN POLYMER JOURNAL
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectCorneal stroma
dc.subjectChitosan
dc.subjectMesenchymal stem cell
dc.subjectPolyvinyl-alcohol
dc.subject3D printing
dc.subjectCHITOSAN
dc.subjectSCAFFOLD
dc.subjectDEGRADATION
dc.subjectTHICKNESS
dc.subjectHYDROGELS
dc.subjectBEHAVIOR
dc.subjectFILMS
dc.title3D printed artificial cornea for corneal stromal transplantation
dc.typearticle
dspace.entity.typePublication
local.avesis.idf68f3760-1f6c-437c-a417-a52209e947c0
local.import.packageSS16
local.indexed.atWOS
local.indexed.atSCOPUS
local.journal.articlenumber109744
local.journal.numberofpages12
local.journal.quartileQ1
oaire.citation.titleEUROPEAN POLYMER JOURNAL
oaire.citation.volume133
relation.isAuthorOfPublicationdda71138-8ce4-4265-89b2-73bc94786a4f
relation.isAuthorOfPublication.latestForDiscoverydda71138-8ce4-4265-89b2-73bc94786a4f

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