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Production and characterization of a high molecular weight levan and fructooligosaccharides from a rhizospheric isolate of Bacillus aryabhattai

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dc.contributor.authorTOKSOY ÖNER, EBRU
dc.contributor.authorsNasir, Anam; Sattar, Fazal; Ashfaq, Iram; Lindemann, Stephen R.; Chen, Ming-Hsu; Van den Ende, Wim; Oner, Ebru Toksoy; Kirtel, Onur; Khaliq, Shazia; Ghauri, M. Afzal; Anwar, Munir A.
dc.date.accessioned2022-03-14T09:19:33Z
dc.date.available2022-03-14T09:19:33Z
dc.date.issued2020-04
dc.description.abstractBacillus aryabhattai GYC2-3 strain, capable of synthesizing EPS, was isolated from the rhizosphere of Taraxacum spp. plant. The EPS was identified as levan by C-13 NMR spectroscopy and methylation analysis. The levan produced had a high weight average molecular weight of 5.317 x 10(7) Da and degree of branching 5.19%. Thin layer chromatography and high-performance anion-exchange chromatography revealed that it is capable of producing broad range of fructooligosaccharides (FOS) and higher levels of FOS synthesis were observed with increasing sucrose concentrations. The optimum temperature, initial pH and sucrose concentration for levan biosynthesis were studied to be 30 degrees C, 8.0 and 250 g/L, respectively. Levan production was increased by similar to 38% (26 g/L) when provided aeration as compare to static cultures. To the best of our knowledge, this is the first report on biosynthesis of levan and FOS by a B. aryabhattai strain.
dc.identifier.doi10.1016/j.lwt.2020.109093
dc.identifier.eissn1096-1127
dc.identifier.issn0023-6438
dc.identifier.urihttps://hdl.handle.net/11424/242965
dc.identifier.wosWOS:000525726500032
dc.language.isoeng
dc.publisherELSEVIER
dc.relation.ispartofLWT-FOOD SCIENCE AND TECHNOLOGY
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectBacillus aryabhattai
dc.subjectLevan
dc.subjectFructooligosaccharides
dc.subjectExopolysaccharide
dc.subjectPrebiotics
dc.subjectSTRUCTURAL-CHARACTERIZATION
dc.subjectFRUCTANSUCRASE ENZYMES
dc.subjectLEVANSUCRASE
dc.subjectOPTIMIZATION
dc.subjectSTRAIN
dc.titleProduction and characterization of a high molecular weight levan and fructooligosaccharides from a rhizospheric isolate of Bacillus aryabhattai
dc.typearticle
dspace.entity.typePublication
local.avesis.idb88a11d6-c636-4891-87f3-6283b762d521
local.import.packageSS16
local.indexed.atWOS
local.indexed.atSCOPUS
local.journal.articlenumber109093
local.journal.numberofpages8
local.journal.quartileQ1
oaire.citation.titleLWT-FOOD SCIENCE AND TECHNOLOGY
oaire.citation.volume123
relation.isAuthorOfPublication6118e9e4-a58e-429b-bbec-4f73a3089a2b
relation.isAuthorOfPublication.latestForDiscovery6118e9e4-a58e-429b-bbec-4f73a3089a2b

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