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Treatment with estrogen receptor agonist ER beta improves torsion-induced oxidative testis injury in rats

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dc.contributor.authorYILDIRIM, ALPER
dc.contributor.authorsTamer, Sevil Arabaci; Yildirim, Alper; Arabaci, Sule; Ciftci, Selin; Akin, Sena; Sari, Elif; Koroglu, M. Kutay; Ercan, Feriha; Yuksel, Meral; Cevik, Ozge; Yegen, Berrak C.
dc.date.accessioned2022-03-12T22:38:12Z
dc.date.available2022-03-12T22:38:12Z
dc.date.issued2019
dc.description.abstractAims: The purpose of the present study was to investigate the potential antioxidant, anti-apoptotic and sperm function-preserving effects of estrogen, estrogen receptor (ER) a and ER beta agonists in a rat model of testis torsion-detorsion (T/D). Main methods: Under anesthesia, 6-8-week-old male Sprague-Dawley rats underwent sham-operation or testicular torsion by fixing left testis rotated at 720 degrees for 2 h. After detorsion, rats were treated with ER alpha agonist (1 mg/kg/day, subcutaneously, sc) or ER beta agonist (1 mg/kg/day, sc) or estradiol (E-2, 1 mg/kg/day, in drinking water) or vehicle on the following two days. On the third day, testicular blood-flow was recorded and then left testes were extracted for molecular and histochemical analysis. Key findings: The findings showed that reduced testicular blood-flow following torsion was partially restored on the 3rd day of detorsion, while treatments with either of the ER agonists or E-2 returned blood flow fully back to the control levels. When the testis-torsioned rats were given ER beta agonist during the detorsion period, tubular injury was lessened, sperm count and motility were increased, while the production of reactive oxygen meta-bolites and apoptosis in the testis tissues were totally suppressed. Although a down-regulated expression of androgen receptor (AR) along with a reduction in serum testosterone level was observed in the vehicle-treated T/D group, all three treatments up-regulated the expressions of AR and its mRNA, while ER alpha agonist and E-2 suppressed the testosterone level. Significance: ER beta receptor activation during the post-ischemic period may be beneficial in protection against torsion-related oxidant testicular injury and infertility.
dc.identifier.doi10.1016/j.lfs.2019.02.056
dc.identifier.eissn1879-0631
dc.identifier.issn0024-3205
dc.identifier.pubmed30825546
dc.identifier.urihttps://hdl.handle.net/11424/235540
dc.identifier.wosWOS:000461421000025
dc.language.isoeng
dc.publisherPERGAMON-ELSEVIER SCIENCE LTD
dc.relation.ispartofLIFE SCIENCES
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectTesticular torsion
dc.subjectEstrogen
dc.subjectInfertility
dc.subjectSperm
dc.subjectApoptosis
dc.subjectOxidative injury
dc.subjectISCHEMIA-REPERFUSION
dc.subjectTESTICULAR TORSION
dc.subjectGENE-EXPRESSION
dc.subjectNITRIC-OXIDE
dc.subjectALPHA
dc.subjectMECHANISMS
dc.subjectAROMATASE
dc.subjectPARAMETERS
dc.subjectAPOPTOSIS
dc.subjectLIGANDS
dc.titleTreatment with estrogen receptor agonist ER beta improves torsion-induced oxidative testis injury in rats
dc.typearticle
dspace.entity.typePublication
local.avesis.id1023bc54-aa23-4fdb-9b6e-d9614b384065
local.import.packageSS17
local.indexed.atWOS
local.indexed.atSCOPUS
local.indexed.atPUBMED
local.journal.numberofpages9
local.journal.quartileQ2
oaire.citation.endPage211
oaire.citation.startPage203
oaire.citation.titleLIFE SCIENCES
oaire.citation.volume222
relation.isAuthorOfPublication1840e6a0-5ec8-42b9-bca3-4e891749ac77
relation.isAuthorOfPublication.latestForDiscovery1840e6a0-5ec8-42b9-bca3-4e891749ac77

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