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Effects of cholinergic compounds and TNF-alpha on human erythroleukemia K562 cell proliferation and caspase expression

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dc.contributor.authorAYDIN OMAY, BANU
dc.contributor.authorsKanli, Zebra; Aydin, Banu; Cabadak, Hulya
dc.date.accessioned2022-03-14T09:08:09Z
dc.date.available2022-03-14T09:08:09Z
dc.date.issued2019-01-31
dc.description.abstractObjective: The purpose of this study was to investigate if stimulating auto-paracrine muscarinic receptor signalling pathway could change human erythroleukemia K562 cell proliferation and caspase 3, 8 and 9 expression levels. To better understand the role of muscarinic receptors in cell signalling mechanism, we investigated the effects of several compounds on human erythroleukemia K562 cell proliferation and caspase 3, 8 and 9 expression. These compounds were M-3 muscarinic receptor agonist, pilocarpine, pro-inflammatory cytokine, tumor necrosis factor (TNF)-alpha, and the wortmannin which is a phosphoinositide 3-kinase inhibitor. Materials and Methods: Cell proliferation and cell viability were evaluated by the trypan blue exclusion test and 5-Bromo-2-deoxy-uridine (BrdU) Labelling and Detection Kits. Caspase 3, 8 and 9 expression levels were determined by immunoblot analysis. Results: Both pilocarpine and TNF-alpha caused a small increase in human erythroleukemia K562 cell proliferation. However, when all the compounds were treated together, proliferation of human erythroleukemia K562 cells increased significantly when compared to untreated control cells. TNF-alpha and wortmannin treatment increased caspase 3 and caspase 8 expression patterns significantly in human erythroleukemia K562 cells. TNF-alpha and wortmannin treatment increased caspase 9 expression level (P>0.05) but it was not significant. Conclusion: These findings partly demonstrated that M-3 muscarinic receptor mediated an increase in K562 cell proliferation. Pilocarpine prevented TNF-alpha and wortmannin induced caspase 3 and 8 expression and indirectly showed apoptosis in human erythroleukemia K562 cells.
dc.identifier.doi10.5472/marumj.518797
dc.identifier.issn1309-9469
dc.identifier.urihttps://hdl.handle.net/11424/242608
dc.identifier.wosWOS:000457122100004
dc.language.isoeng
dc.publisherMARMARA UNIV, FAC MEDICINE
dc.relation.ispartofMARMARA MEDICAL JOURNAL
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectM-3 muscarinic receptors
dc.subjectCytokine
dc.subjectPilocarpine
dc.subjectCaspases
dc.subjectErythroleukemia K562 cells
dc.subjectTUMOR-NECROSIS-FACTOR
dc.subjectSIGNAL-TRANSDUCTION
dc.subjectAPOPTOSIS
dc.subjectACETYLCHOLINE
dc.subjectACTIVATION
dc.subjectRECEPTORS
dc.subjectKINASE
dc.titleEffects of cholinergic compounds and TNF-alpha on human erythroleukemia K562 cell proliferation and caspase expression
dc.typearticle
dspace.entity.typePublication
local.avesis.id39eebdcd-bc74-4e96-913c-f8fa3427443d
local.import.packageSS16
local.indexed.atWOS
local.indexed.atSCOPUS
local.indexed.atTRDIZIN
local.journal.numberofpages7
oaire.citation.endPage26
oaire.citation.issue1
oaire.citation.startPage20
oaire.citation.titleMARMARA MEDICAL JOURNAL
oaire.citation.volume32
relation.isAuthorOfPublicationaf9e48cc-cef6-4af2-bdb7-5b424aadfc2b
relation.isAuthorOfPublication.latestForDiscoveryaf9e48cc-cef6-4af2-bdb7-5b424aadfc2b

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