Publication:
Effect of whey protein derivatives on cell viability, cell migration and cell cycle phases in MCF-7 cells

Simple item page
dc.contributor.authorYILMAZ GÖLER, AYŞE MİNE
dc.contributor.authorYALÇIN, AHMET SUHA
dc.contributor.authorsAksoy F. T., YILMAZ GÖLER A. M., Bicim G., Yalcin A. S.
dc.date.accessioned2023-03-13T06:29:36Z
dc.date.available2023-03-13T06:29:36Z
dc.date.issued2023-01-01
dc.description.abstractObjective: This study aimed to obtain protein derivatives after treatment of whey proteins with hazelnut oil and olive oil and determined their effects on MCF-7 cells. Materials and Methods: Whey proteins obtained from 6% whey powder were treated with hazelnut oil (HO) and olive oil (OO) at a protein to lipid ratio of 1:10 at 60 ̊C for 120 minutes. The protein derivatives formed with whey protein and HO or OO were applied to MCF-7 cancer cells and healthy fibroblasts. The effects of protein derivatives on cell viability, apoptosis, reactive oxygen species (ROS) production, wound healing, cell cycle phase distribution and cell cycle related proteins Akt and p21(Waf1/Cip1) expressions were investigated. Results: Cell viability decreased significantly after 24 h of incubation with WP:OO. The percentage of apoptotic or necrotic cells varied between 5-10% and no statistically significant effect was observed. There was no statistically significant difference in ROS production and colony formation between controls and WP:HO or WP:OO groups. Treatment of cells with WP:OO for 24 h significantly decreased cell migration compared to the control group. G2/M phase was significantly suppressed in WP:OO group compared to the control group. WP:OO also increased the expression of p21(Waf1/Cip1) significantly when compared with the control group. Conclusion: Our results showed that whey protein derivatives applied to MCF-7 cells are cytotoxic and may be useful in breast cancer treatment
dc.identifier.citationAksoy F. T., YILMAZ GÖLER A. M., Bicim G., Yalcin A. S., "Effect of whey protein derivatives on cell viability, cell migration and cell cycle phases in MCF-7 cells", MARMARA MEDICAL JOURNAL, cilt.36, sa.1, ss.39-45, 2023
dc.identifier.doi10.5472/marumj.1244676
dc.identifier.endpage45
dc.identifier.issn1309-9469
dc.identifier.issue1
dc.identifier.startpage39
dc.identifier.urihttps://avesis.marmara.edu.tr/api/publication/49d863cd-99a1-4a78-abdd-fec97967e8b2/file
dc.identifier.urihttps://hdl.handle.net/11424/287372
dc.identifier.volume36
dc.language.isoeng
dc.relation.ispartofMARMARA MEDICAL JOURNAL
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectTıp
dc.subjectSağlık Bilimleri
dc.subjectTemel Tıp Bilimleri
dc.subjectMedicine
dc.subjectHealth Sciences
dc.subjectFundamental Medical Sciences
dc.subjectTIP, GENEL & DAHİLİ
dc.subjectKlinik Tıp
dc.subjectKlinik Tıp (MED)
dc.subjectMEDICINE, GENERAL & INTERNAL
dc.subjectCLINICAL MEDICINE
dc.subjectClinical Medicine (MED)
dc.subjectGenel Sağlık Meslekleri
dc.subjectPatofizyoloji
dc.subjectTemel Bilgi ve Beceriler
dc.subjectDeğerlendirme ve Teşhis
dc.subjectDahiliye
dc.subjectAile Sağlığı
dc.subjectTıp (çeşitli)
dc.subjectGenel Tıp
dc.subjectGeneral Health Professions
dc.subjectPathophysiology
dc.subjectFundamentals and Skills
dc.subjectAssessment and Diagnosis
dc.subjectInternal Medicine
dc.subjectFamily Practice
dc.subjectMedicine (miscellaneous)
dc.subjectGeneral Medicine
dc.subjectWhey proteins
dc.subjectOleic acid
dc.subjectCell survival
dc.subjectApoptosis
dc.subjectCell cycle
dc.subjectHUMAN ALPHA-LACTALBUMIN
dc.subjectOLEIC-ACID
dc.subjectOLIVE OIL
dc.subjectCYTOTOXIC COMPLEXES
dc.subjectTUMOR-CELLS
dc.subjectHAMLET
dc.subjectCANCER
dc.subjectBOVINE
dc.subjectLETHAL
dc.subjectAPOPTOSIS
dc.subjectWhey proteins
dc.subjectOleic acid
dc.subjectCell survival
dc.subjectApoptosis
dc.subjectCell cycle
dc.titleEffect of whey protein derivatives on cell viability, cell migration and cell cycle phases in MCF-7 cells
dc.typearticle
dspace.entity.typePublication
local.avesis.id49d863cd-99a1-4a78-abdd-fec97967e8b2
local.indexed.atWOS
relation.isAuthorOfPublication8a72311b-9078-47c0-9fd8-bd73292b7739
relation.isAuthorOfPublication7a2bc583-aab6-43e7-9cc3-0d7a60773409
relation.isAuthorOfPublication.latestForDiscovery8a72311b-9078-47c0-9fd8-bd73292b7739

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
12.pdf
Size:
760.27 KB
Format:
Adobe Portable Document Format
Download

Collections

Research Outputs