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Regulation of M-2, M-3, and M-4 muscarinic receptor expression in K562 chronic myelogenous leukemic cells by carbachol

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dc.contributor.authorAYDIN OMAY, BANU
dc.contributor.authorsCabadak, Hulya; Aydin, Banu; Kan, Beki
dc.date.accessioned2022-03-12T17:52:28Z
dc.date.available2022-03-12T17:52:28Z
dc.date.issued2011
dc.description.abstractContext: Muscarinic receptors mediate a variety of cellular responses to acetylcholine, including inhibition of adenylate cyclase, breakdown of phosphoinositide and modulation of ion channels. These receptors are relatively abundant in the central nervous system and peripheral parasympathetic nervous system. Many cells express a mixture of muscarinic receptor transcripts. Changes in muscarinic M-2 and M-3 receptor mRNA levels in response to agonist treatment have been reported in cerebellar granule cells, Chinese hamster ovary cells, lymphocytes and in the human neuroblastoma cell line SH-SY5Y. Objective: In this study, we investigated the effects of agonist stimulation on cell proliferation and on the levels of muscarinic receptor expression in K562 chronic myelogenous leukemia cells. Methods:Total RNA and crude membrane fractions were prepared from K562 cells challenged with carbachol (CCh). Muscarinic receptor subtype expression was determined by RT-PCR and western blot analysis. Proliferation and cell viability were evaluated by the trypan blue exclusion test and BrDU labeling. Results: We showed that CCh-treatment leads to changes in muscarinic M-2, M-3, and M-4 receptor transcripts as well as M-2 and M-3 protein levels. We also found that CCh decreased proliferation of K562 cells in a time dependent manner, an effect prevented by atropine. These results suggest that CCh modulates K562 chronic myelogenous leukemic cells proliferation through muscarinic acetylcholine receptors.
dc.identifier.doi10.3109/10799893.2010.506484
dc.identifier.issn1079-9893
dc.identifier.pubmed20684668
dc.identifier.urihttps://hdl.handle.net/11424/230406
dc.identifier.wosWOS:000287775900004
dc.language.isoeng
dc.publisherINFORMA HEALTHCARE
dc.relation.ispartofJOURNAL OF RECEPTORS AND SIGNAL TRANSDUCTION
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectAgonist
dc.subjectcell proliferation
dc.subjectmRNA regulation
dc.subjectINDUCED DOWN-REGULATION
dc.subjectCEREBELLAR GRANULE CELLS
dc.subjectMESSENGER-RNA EXPRESSION
dc.subjectACETYLCHOLINE-RECEPTOR
dc.subjectCHOLINERGIC SYSTEM
dc.subjectDNA-SYNTHESIS
dc.subjectSUBTYPES
dc.subjectLINE
dc.subjectACTIVATION
dc.subjectIDENTIFICATION
dc.titleRegulation of M-2, M-3, and M-4 muscarinic receptor expression in K562 chronic myelogenous leukemic cells by carbachol
dc.typearticle
dspace.entity.typePublication
local.avesis.idf8ce094f-99cf-4dca-8c4e-4a303de2ec79
local.import.packageSS17
local.indexed.atWOS
local.indexed.atSCOPUS
local.indexed.atPUBMED
local.journal.numberofpages7
oaire.citation.endPage32
oaire.citation.issue1
oaire.citation.startPage26
oaire.citation.titleJOURNAL OF RECEPTORS AND SIGNAL TRANSDUCTION
oaire.citation.volume31
relation.isAuthorOfPublicationaf9e48cc-cef6-4af2-bdb7-5b424aadfc2b
relation.isAuthorOfPublication.latestForDiscoveryaf9e48cc-cef6-4af2-bdb7-5b424aadfc2b

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