Publication:
Optimization of ethanol production from starch by an amylolytic nuclear petite Saccharomyces cerevisiae strain

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dc.contributor.authorTOKSOY ÖNER, EBRU
dc.contributor.authorsOner, Ebru Toksoy
dc.date.accessioned2022-03-12T17:18:57Z
dc.date.available2022-03-12T17:18:57Z
dc.date.issued2006
dc.description.abstractEthanol fermentation characteristics of the 100% respiratory-deficient nuclear petite amylolytic Saccharomyces cerevisiae NPB-G strain was investigated in both shake-flask and controlled bioreactor cultivation conditions, and comparison with the earlier reported results revealed 54.6% increase in ethanol yield. Efforts to improve the starch utilization rate by increasing the selection pressure or supplying the fermentation medium with glucose did not prevent the observed decrease in time-dependent amylolytic activity. Response surface methodology (RSM) was then used as a statistical tool to optimize the initial yeast extract and starch contents of the medium, which resulted in a substantial increase in the stability of the expression plasmid in both the respiratory-deficient NPB-G and the parental respiratory-sufficient WTPB-G strains, with concomitant improvement in their amylolytic potentials. High ethanol yields on substrate values of the bioreactor cultures, which were very close to the theoretical yield, indicated that the amylolytic respiratory-deficient NPB-G strain was effective in the direct fermentation of starch into ethanol. Copyright (c) 2006 John Wiley & Sons, Ltd.
dc.identifier.doi10.1002/yea.1399
dc.identifier.eissn1097-0061
dc.identifier.issn0749-503X
dc.identifier.pubmed17001624
dc.identifier.urihttps://hdl.handle.net/11424/228035
dc.identifier.wosWOS:000242009800001
dc.language.isoeng
dc.publisherWILEY
dc.relation.ispartofYEAST
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectyeast
dc.subjectoptimization
dc.subjectethanol
dc.subjectstarch
dc.subjectfermentation
dc.subjectnuclear petite
dc.subjectLIPOMYCES-KONONENKOAE
dc.subjectEFFICIENT UTILIZATION
dc.subjectRECOMBINANT YEAST
dc.subjectALPHA-AMYLASE
dc.subjectCORN STARCH
dc.subjectGLUCOAMYLASE
dc.subjectFERMENTATION
dc.subjectIMPROVEMENT
dc.subjectALCOHOL
dc.subjectGENES
dc.titleOptimization of ethanol production from starch by an amylolytic nuclear petite Saccharomyces cerevisiae strain
dc.typearticle
dspace.entity.typePublication
local.avesis.id2a0b23f6-6ef4-4d5c-b273-396fb1d6c589
local.import.packageSS17
local.indexed.atWOS
local.indexed.atSCOPUS
local.indexed.atPUBMED
local.journal.numberofpages8
oaire.citation.endPage856
oaire.citation.issue12
oaire.citation.startPage849
oaire.citation.titleYEAST
oaire.citation.volume23
relation.isAuthorOfPublication6118e9e4-a58e-429b-bbec-4f73a3089a2b
relation.isAuthorOfPublication.latestForDiscovery6118e9e4-a58e-429b-bbec-4f73a3089a2b

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