Publication:
Thyroxine prevents reoxygenation injury in isolated proximal tubule cells

Simple item page
dc.contributor.authorYALÇIN, AHMET SUHA
dc.contributor.authorsErkan, E; Sakarcan, A; Haklar, G; Yalcin, S
dc.date.accessioned2022-03-12T17:17:05Z
dc.date.available2022-03-12T17:17:05Z
dc.date.issued2003
dc.description.abstractIschemia is characterized by cessation of blood flow and oxygen delivery to tissues that results in disruption of cellular structure and organelles. However, restoration of blood flow following ischemia causes reperfusion injury, characterized by further damage in the tissues mediated by reactive oxygen species. In the kidney, reactive oxygen molecules have been implicated in ischemic, toxic and immunological glomerular damage. Thyroxine has been shown to be cytoprotective in toxic and ischemic injury. Thyroxine's cytoprotective effect is postulated to be secondary to stimulation of intracellular ATP synthesis. However, the underlying mechanism of that beneficial effect remains to be investigated. In this study we investigated the effect of thyroxine (T4) on free oxygen radical production in an in vitro model of reperfusion injury. Free oxygen radical (FOR) levels were determined by a chemiluminescence method after freshly isolated rabbit proximal tubule cells were subjected to 15 min of anoxia followed by 45 min of reoxygenation. Reoxygenation injury resulted in a significant increase in FOR levels (P<0.0001). FOR levels were significantly lower in the group treated with thyroxine (P=0.01) and cells treated with thyroxine displayed better preservation of cellular structure. We conclude that thyroxine's cytoprotective effect might be via decreased synthesis of FOR, and thyroxine treatment may confer cytoprotection in renal conditions characterized by FOR-mediated injury.
dc.identifier.doi10.1007/s00467-003-1123-3
dc.identifier.eissn1432-198X
dc.identifier.issn0931-041X
dc.identifier.pubmed12734748
dc.identifier.urihttps://hdl.handle.net/11424/227765
dc.identifier.wosWOS:000183460700004
dc.language.isoeng
dc.publisherSPRINGER
dc.relation.ispartofPEDIATRIC NEPHROLOGY
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectanoxia-reoxygenation
dc.subjectcellular injury
dc.subjectfree oxygen radicals
dc.subjectthyroxine
dc.subjectACUTE-RENAL-FAILURE
dc.subjectISCHEMIA-REPERFUSION INJURY
dc.subjectOXYGEN FREE-RADICALS
dc.subjectEPITHELIAL-CELLS
dc.subjectXANTHINE-OXIDASE
dc.subjectSUPEROXIDE-DISMUTASE
dc.subjectRAT
dc.subjectDAMAGE
dc.subjectRABBIT
dc.subjectKIDNEYS
dc.titleThyroxine prevents reoxygenation injury in isolated proximal tubule cells
dc.typearticle
dspace.entity.typePublication
local.avesis.id5f48006f-811f-40da-a04f-9dcbdbbe1e4f
local.import.packageSS17
local.indexed.atWOS
local.indexed.atSCOPUS
local.indexed.atPUBMED
local.journal.numberofpages8
oaire.citation.endPage643
oaire.citation.issue7
oaire.citation.startPage636
oaire.citation.titlePEDIATRIC NEPHROLOGY
oaire.citation.volume18
relation.isAuthorOfPublication7a2bc583-aab6-43e7-9cc3-0d7a60773409
relation.isAuthorOfPublication.latestForDiscovery7a2bc583-aab6-43e7-9cc3-0d7a60773409

Files

Collections

Research Outputs