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The methanolic extract of Thymus praecox subsp. skorpilii var. skorpilii restores glucose homeostasis, ameliorates insulin resistance and improves pancreatic beta-cell function on streptozotocin/nicotinamide-induced type 2 diabetic rats

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dc.contributor.authorYAVUZ, AYŞE NUR
dc.contributor.authorsCam, Muhammet Emin; Hazar-Yavuz, Ayse Nur; Yildiz, Sila; Ertas, Busra; Adakul, Betul Ayaz; Taskin, Turgut; Alan, Saadet; Kabasakal, Levent
dc.date.accessioned2022-03-12T22:38:49Z
dc.date.available2022-03-12T22:38:49Z
dc.date.issued2019
dc.description.abstractEthnopharmacological relevance: Thymus praecox subsp. skorpilii var. skorpilii (syn. Thymus praecox subsp. jankae (Celak.) Jalas) is consumed as a Turkish folk medicine for the treatment of spasm, sore throat and shortness of breath, also having strong antioxidant activity and the leaves of the plant have been utilized for the treatment of diabetes as the decoction in Turkey. Aim of the study: In the present study, we aimed to investigate the potential mechanism of antidiabetic action of Thymus praecox subsp. skorpilii var. skorpilii methanolic extract (TPSE) on streptozotocin (STZ)/nicotinamide (NA)-induced type 2 diabetic rats. Materials and methods: Sprague Dawley rats were randomly divided into four groups; control, diabetes, TPSE (100 mg/kg b.w, p.o.) and metformin group (400 mg/kg b.w, p.o.). Diabetes was established in all groups except control group by 55 mg/kg STZ (i.p.) for once 15 min after 100 mg/kg NA injection. 3 days after STZ/NA injection, treatments were administered for three weeks and then rats were decapitated; tissue and blood samples were obtained for measuring the level of glucose transporters (both GLUTs and sodium glucose co-transporters (SGLTs)), enzymes related to glucose (Hexokinase (HK), phosphoenolpyruvate carboxykinase (PEPCK), alpha-glucosidase) and lipid metabolism (Acetyl-coenzyme carboxylase (ACC)), AST, ALT, creatinine, insulin, anti-inflammatory (IL-10) and inflammatory (TNF-alpha, IL-1 beta, IL-6) cytokines, AMP-activated protein kinase (AMPK), peroxisome proliferator-activated receptor gamma (PPAR-gamma) and glucagon like peptide-1 (GLP-1). Histopathological alterations of the pancreas were examined. Results: After three weeks of treatment, TPSE has exhibited a significant reduction of plasma levels of the proinflammatory cytokines. Besides, TPSE treatment elevated plasma insulin levels and normalized blood glucose levels. Moreover, it improved the values of AMPK in liver and GLP-1 in pancreas. Increased a-glucosidase, PEPCK, GLUT-2 and SGLTs levels with the induction of diabetes considerably lowered with TPSE treatment. Especially on SGLT-2, TPSE achieved a more prominent decrease. After the atrophy in Langerhans islets due to
dc.identifier.doi10.1016/j.jep.2018.10.028
dc.identifier.eissn1872-7573
dc.identifier.issn0378-8741
dc.identifier.pubmed30399410
dc.identifier.urihttps://hdl.handle.net/11424/235732
dc.identifier.wosWOS:000455692600004
dc.language.isoeng
dc.publisherELSEVIER IRELAND LTD
dc.relation.ispartofJOURNAL OF ETHNOPHARMACOLOGY
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectDiabetes
dc.subjectMetabolism
dc.subjectInflammation
dc.subjectThymus praecox
dc.subjectSTZ/NA
dc.subjectMedicinal plants
dc.subjectGLUTs
dc.subjectSGLTs
dc.subjectAMPK
dc.subjectPROXIMAL TUBULAR CELLS
dc.subjectGLUT2 GENE-EXPRESSION
dc.subjectIN-VITRO
dc.subjectCARAMANICUS JALAS
dc.subjectESSENTIAL OIL
dc.subjectSODIUM
dc.subjectHYPERGLYCEMIA
dc.subjectTRANSPORTERS
dc.subjectCOTRANSPORTER
dc.subjectINTESTINE
dc.titleThe methanolic extract of Thymus praecox subsp. skorpilii var. skorpilii restores glucose homeostasis, ameliorates insulin resistance and improves pancreatic beta-cell function on streptozotocin/nicotinamide-induced type 2 diabetic rats
dc.typearticle
dspace.entity.typePublication
local.avesis.id0f47cea8-eb79-45c8-bb0d-bcd0daee3e16
local.import.packageSS17
local.indexed.atWOS
local.indexed.atSCOPUS
local.indexed.atPUBMED
local.journal.numberofpages10
local.journal.quartileQ1
oaire.citation.endPage38
oaire.citation.startPage29
oaire.citation.titleJOURNAL OF ETHNOPHARMACOLOGY
oaire.citation.volume231
relation.isAuthorOfPublication0bd18078-950c-4d8c-93f6-120ce419897d
relation.isAuthorOfPublication.latestForDiscovery0bd18078-950c-4d8c-93f6-120ce419897d

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