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UV-curable methacrylated/fumaric acid modified epoxy as a potential support for enzyme immobilization

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dc.contributor.authorKAHRAMAN, MEMET VEZİR
dc.contributor.authorsKahraman, M. Vezir; Bayramoglu, Gulay; Kayaman-Apohan, Nilhan; Gungor, Atilla
dc.date.accessioned2022-03-12T17:32:29Z
dc.date.available2022-03-12T17:32:29Z
dc.date.issued2007
dc.description.abstractThe immobilization procedure of UV-curing coating is simple and causes less loss of enzymatic activity. UV-curable methacrylated/fumaric acid modified cycloaliphatic epoxide is here proposed as a rigid support material for covalent immobilization of a-amylase. The immobilized enzyme is analyzed in terms of bioactivity retention as a function of repeated use ability, pH, storage, as well as stability under various experimental conditions, taking starch as a substrate. The properties of immobilized enzyme were also compared with those of the free enzyme. The highest activity of free enzyme was obtained at pH 7.0 while this value was shifted to PH 7.5 for immobilized system. Optimum catalytic activity was observed at 30 degrees C, for both free and immobilized enzyme; however, the immobilized enzyme had a higher activity than the free one. The immobilized enzyme that was used 35 times in 8 h in repeated batch experiments demonstrated that about 73% of the enzyme activity was retained. The free enzyme lost all its activity with in 15 days. The retained activity of immobilized enzyme was found to be around 80%. The amount of bound alpha-amylase was found 94 mg per gram polymeric support material. (c) 2006 Published by Elsevier B.V.
dc.identifier.doi10.1016/j.reactfunctpolym.2006.09.005
dc.identifier.eissn1873-166X
dc.identifier.issn1381-5148
dc.identifier.urihttps://hdl.handle.net/11424/228593
dc.identifier.wosWOS:000244212600002
dc.language.isoeng
dc.publisherELSEVIER
dc.relation.ispartofREACTIVE & FUNCTIONAL POLYMERS
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectcyclo aliphatic epoxide
dc.subjectUV-curing coating
dc.subjectimmobilization
dc.subjectalpha-amylase
dc.subjectenzyme activity
dc.subjectALPHA-AMYLASE
dc.subjectMEMBRANE
dc.subjectUREASE
dc.titleUV-curable methacrylated/fumaric acid modified epoxy as a potential support for enzyme immobilization
dc.typearticle
dspace.entity.typePublication
local.avesis.id3ae4567f-f218-43e8-b154-f575360ff4fa
local.import.packageSS17
local.indexed.atWOS
local.indexed.atSCOPUS
local.journal.numberofpages7
oaire.citation.endPage103
oaire.citation.issue2
oaire.citation.startPage97
oaire.citation.titleREACTIVE & FUNCTIONAL POLYMERS
oaire.citation.volume67
relation.isAuthorOfPublication7676f3ad-0384-4001-b17d-b928ae2de7e6
relation.isAuthorOfPublication.latestForDiscovery7676f3ad-0384-4001-b17d-b928ae2de7e6

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