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Melatonin ameliorates ionizing radiation-induced oxidative organ damage in rats

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dc.contributor.authorATASOY, BESTE MELEK
dc.contributor.authorsSener, G; Jahovic, N; Tosun, O; Atasoy, BM; Yegen, BC
dc.date.accessioned2022-03-12T17:16:46Z
dc.date.available2022-03-12T17:16:46Z
dc.date.issued2003
dc.description.abstractThis study was designed to study the effects of the potential radioprotective properties of pharmacological doses of melatonin against organ damage induced by whole-body irradiation (IR) in rats. A total of 32 male Sprague-Dawley rats were exposed to irradiation performed with a LINAC producing 6 MV photons at a focus 100 cm distant from the skin. Under ketamine anaesthesia, each rat received a single whole-body dose of 800 cGy. Immediately before and after IR, rats were treated with either saline or melatonin (20 mg/kg and 10 mg/kg, ip) and decapitated at 12-h after exposure to irradiation. Another group of rats was followed for 72-h after IR, where melatonin (10 mg/kg, ip) injections were repeated once daily. Tissue levels of malondialdehyde (MDA)an index of lipid peroxidation-, glutathione (GSH)-a key to antioxidant- and myeloperoxidase (MPO) activity-an index of neutrophil infiltration-were estimated in liver, lung, colon and intestinal tissues. The results demonstrate that both 12-h and 72-h following IR, tissue leves of MDA were elevated (p<0.05-0.001), while GSH levels were reduced (p<0.05-0.001) in all organs. On the other hand, melatonin, reduced the levels of MDA and increased the GSH levels significantly, (p<0.05-0.001). MPO activity was increased significantly in the colonic tissue at the both 12-h and 72-h, and in the hepatic tissue at the 72-h following IR, which were reduced by melatonin (p<0.01-0.001). In the lung tissue enzyme activity was decreased at 72nd h of postirradiation. In conclusion, the increase in MDA levels and MPO activity and the concomitant decrease in GSH levels demonstrate the role of oxidative mechanisms in irradiation-induced tissue damage, and melatonin, by its free radical scavenging and antioxidant properties, ameliorates irradiation-induced organ injury. Thus, supplementing cancer patients with adjuvant therapy of melatonin may have some benefit for successful radiotherapy. (C) 2003 Elsevier Inc. All rights reserved.
dc.identifier.doi10.1016/j.lfs.2003.05.011
dc.identifier.eissn1879-0631
dc.identifier.issn0024-3205
dc.identifier.pubmed14623027
dc.identifier.urihttps://hdl.handle.net/11424/227684
dc.identifier.wosWOS:000186809900003
dc.language.isoeng
dc.publisherPERGAMON-ELSEVIER SCIENCE LTD
dc.relation.ispartofLIFE SCIENCES
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectirradiation
dc.subjectlipid peroxidation
dc.subjectglutathione
dc.subjectmyeloperoxidase
dc.subjectLIPID-PEROXIDATION
dc.subjectMYELOPEROXIDASE
dc.subjectGLUTATHIONE
dc.subjectMECHANISMS
dc.subjectSTRESS
dc.titleMelatonin ameliorates ionizing radiation-induced oxidative organ damage in rats
dc.typearticle
dspace.entity.typePublication
local.avesis.id01643f37-779f-48bd-9e99-a078da46e053
local.import.packageSS17
local.indexed.atWOS
local.indexed.atSCOPUS
local.indexed.atPUBMED
local.journal.numberofpages10
oaire.citation.endPage572
oaire.citation.issue5
oaire.citation.startPage563
oaire.citation.titleLIFE SCIENCES
oaire.citation.volume74
relation.isAuthorOfPublication22ce1b48-93da-4e88-a61e-be24b5e6122a
relation.isAuthorOfPublication.latestForDiscovery22ce1b48-93da-4e88-a61e-be24b5e6122a

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