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The role of nitric oxide in the reversal of hemorrhagic shock by oxotremorine

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dc.contributor.authorAKICI, AHMET
dc.contributor.authorsGören, M. Z.; Akici, A.; Karaalp, A.; Aker, R.; Oktay, S.
dc.date.accessioned2022-03-15T11:26:58Z
dc.date.available2022-03-15T11:26:58Z
dc.date.issued2001
dc.description.abstractIn the present study, the effect of the nitric oxide synthase inhibitor, N(G)-nitro-L-arginine methylester (L-NAME), on the antishock actions of oxotremorine was investigated in rats subjected to hemorrhagic shock under urethane anesthesia. L-citrulline production in the AV3V region, as an indicator of nitric oxide (NO) synthesis, was assayed by high-performance liquid chromatography (HPLC) with fluorescent detection throughout the experiment. The rats were pretreated with either intravenous (i.v.) physiological saline or L-NAME (2.5 mg/kg) before bleeding. L-NAME potentiated the reversal of hypotension by oxotremorine (25 microg/kg, i.v.). However, oxotremorine either alone or in combination with L-NAME did not produce any significant change in 60-min survival rate at this low dose. Analysis of microdialysis samples collected from the AV3V region showed that L-citrulline concentration increased during bleeding and that this increase was abolished by L-NAME pretreatment. These results may suggest that nitric oxide production contributes to hypotension in rats bled to shock since nitric oxide levels in the AV3V region increased in response to bleeding and nitric oxide synthase (NOS) inhibition abolished this increase and potentiated the oxotremorine-induced reversal of hypotension.
dc.identifier.doi10.1016/s0014-2999(01)01294-8
dc.identifier.issn0014-2999
dc.identifier.pubmedPMID: 11675044
dc.identifier.urihttps://hdl.handle.net/11424/250437
dc.language.isoeng
dc.relation.ispartofEuropean Journal of Pharmacology
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectFemale
dc.subjectAnimals
dc.subjectMale
dc.subjectRats
dc.subjectBlood Pressure
dc.subjectTime Factors
dc.subjectNitric Oxide
dc.subjectRats, Sprague-Dawley
dc.subjectSurvival Rate
dc.subjectNitric Oxide Synthase
dc.subjectCerebral Ventricles
dc.subjectEnzyme Inhibitors
dc.subjectNG-Nitroarginine Methyl Ester
dc.subjectShock, Hemorrhagic
dc.subjectOxotremorine
dc.subjectCitrulline
dc.titleThe role of nitric oxide in the reversal of hemorrhagic shock by oxotremorine
dc.typearticle
dspace.entity.typePublication
local.avesis.id03634abc-a1c9-4f1e-933b-825ea4610f9c
local.import.packageSS23
local.import.sourcePubMed
local.indexed.atPUBMED
oaire.citation.endPage267
oaire.citation.startPage261
oaire.citation.titleEuropean Journal of Pharmacology
oaire.citation.volume2
relation.isAuthorOfPublication6c7c9f26-63a9-4f6f-91cd-aa9e3452919c
relation.isAuthorOfPublication.latestForDiscovery6c7c9f26-63a9-4f6f-91cd-aa9e3452919c

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